Kinetic study of substrate dependency for higher butanol production in acetone-butanol-ethanol fermentation

Hideaki Shinto, Yukihiro Tashiro, Genta Kobayashi, Tatsuya Sekiguchi, Taizo Hanai, Yuki Kuriya, Masahiro Okamoto, Kenji Sonomoto

研究成果: ジャーナルへの寄稿記事

51 引用 (Scopus)

抄録

A kinetic simulation model of acetone-butanol-ethanol (ABE) fermentation of xylose (ModelXYL) was proposed by substituting Embden-Meyerhof-Parnas (EMP) pathway equations in the glucose model (ModelGLC) by pentose phosphate (PP) pathway equations of xylose utilization. We estimated the equation parameters of the PP pathway and set other equation parameters to the same as those in ModelGLC, by which ModelXYL exhibited an r2 value of 0.901 between the experimental time course of metabolites with initial xylose concentrations ranging from 40.7 to 292 mM and the calculated values. The results with the developed model suggested that Clostridium saccharoperbutylacetonicum N1-4 has a robust metabolic network in acid- and solvent-producing pathways. Furthermore, sensitivity analysis revealed that slow substrate utilization would be effective for higher butanol production; this coincided with the experimental results. Therefore, we consider the proposed model to be one of the best kinetic simulation candidates describing the dynamic metabolite behavior in ABE production.

元の言語英語
ページ(範囲)1452-1461
ページ数10
ジャーナルProcess Biochemistry
43
発行部数12
DOI
出版物ステータス出版済み - 12 1 2008

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Butanols
Xylose
Acetone
Butenes
Fermentation
Pentoses
Pentose Phosphate Pathway
Ethanol
Metabolites
Kinetics
Phosphates
Substrates
Enzyme kinetics
Clostridium
Glycolysis
Metabolic Networks and Pathways
Sensitivity analysis
Glucose
Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

これを引用

Kinetic study of substrate dependency for higher butanol production in acetone-butanol-ethanol fermentation. / Shinto, Hideaki; Tashiro, Yukihiro; Kobayashi, Genta; Sekiguchi, Tatsuya; Hanai, Taizo; Kuriya, Yuki; Okamoto, Masahiro; Sonomoto, Kenji.

:: Process Biochemistry, 巻 43, 番号 12, 01.12.2008, p. 1452-1461.

研究成果: ジャーナルへの寄稿記事

Shinto, Hideaki ; Tashiro, Yukihiro ; Kobayashi, Genta ; Sekiguchi, Tatsuya ; Hanai, Taizo ; Kuriya, Yuki ; Okamoto, Masahiro ; Sonomoto, Kenji. / Kinetic study of substrate dependency for higher butanol production in acetone-butanol-ethanol fermentation. :: Process Biochemistry. 2008 ; 巻 43, 番号 12. pp. 1452-1461.
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AU - Hanai, Taizo

AU - Kuriya, Yuki

AU - Okamoto, Masahiro

AU - Sonomoto, Kenji

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