Kwakhurin-magnetic particles conjugates enable fast enzyme immunoassay for the detection of kwakhurin in Pueraria candollei

Seiichi Sakamoto, Ryota Eto, Poomraphie Nuntawong, Gorawit Yusakul, Thaweesak Juengwatanatrakul, Waraporn Putalun, Shunsuke Fujii, Hiroyuki Tanaka, Satoshi Morimoto

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

Introduction: Kwakhurin (Kwa) is one of the unique isoflavonoids produced in Pueraria candollei var. mirifica (P. candollei), which has long been used as folk medicine for rejuvenation in Thailand. Recently, the use of P. candollei-derived products has widely spread among Japanese women for cosmetic purposes. Correspondingly, there has been an increase in the number of reports regarding possible health hazards caused by estrogenic activity inherent to the plant; thus, the need for a detailed evaluation of the phytoestrogen content of P. candollei-derived products has gained a sense of urgency in recent years. Objective: This study aims to develop a rapid enzyme immunoassay that can be applied to the quantitative analysis of Kwa in P. candollei and its derived products. Material and Method: A rapid and sensitive immunoassay was developed with a combination of Kwa-specific monoclonal antibody (MAb 11F) and Kwa–magnetic particles (MPs) conjugates, which increased the surface area of the solid phase, resulting in a decrease in the immunoreaction time. Result: This novel MPs-based enzyme immunoassay (MPs-EIA) was used to determine Kwa concentration in the range from 2.44 to 78.1 ng/mL with a limit of detection of 1.90 ng/mL. Validation analyses revealed that the proposed MPs-EIA protocol was sufficiently precise and accurate for effective quantitative analysis of Kwa in P. candollei and its derived products.

本文言語英語
ページ(範囲)930-936
ページ数7
ジャーナルPhytochemical Analysis
31
6
DOI
出版ステータス出版済み - 11 1 2020

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 食品科学
  • 生化学
  • 分子医療
  • 植物科学
  • 創薬
  • 補完代替医療

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