L-serine regulates the activities of microglial cells that express very low level of 3-phosphoglycerate dehydrogenase, an enzyme for L-serine biosynthesis

Hiroki Sugishita, Yasuhide Kuwabara, Kazuko Toku, Lisa Doi, Lihua Yang, Junya Mitoma, Shigeki Furuya, Yoshio Hirabayashi, Nobuji Maeda, Masahiro Sakanaka, Junya Tanaka

研究成果: ジャーナルへの寄稿記事

20 引用 (Scopus)

抄録

Microglia are well known to become activated during various kinds of neuropathological events. The factors that are responsible for the activation, however, are not fully determined. In the present study, L-Ser was shown to enhance production of nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor α (TNF α) by lipopolysaccharide (LPS)-stimulated cultured rat microglial cells. L-Ser, however, did not enhance the expression of mRNAs encoding inducible NO synthase, IL-6 and TNF α. On the other hand, astrocytes did not depend on L-Ser for release of IL-6 and TNF α. The expression of an enzyme 3-phosphoglycerate dehydrogenase (3PGDH), which is essential for L-Ser biosynthesis from a glycolytic intermediate 3-phosphoglycerate, was investigated. As revealed by Western blotting and immunocytochemical staining, 3PGDH-protein expression in vitro was the highest in astrocytes, intermediate in neurons and the lowest in microglial cells. Semiquantitative RT-PCR showed that microglial cells expressed 3PGDH-mRNA at a lower level than astrocytes. In frozen sections from rat forebrain, only astrocytes were immunoreactive for 3PGDH. The present study suggested that L-Ser is able to modulate microglial function mainly at the translation level because microglial cells cannot synthesize sufficient amount of L-Ser due to the scarce expression of 3PGDH.

元の言語英語
ページ(範囲)392-401
ページ数10
ジャーナルJournal of Neuroscience Research
64
発行部数4
DOI
出版物ステータス出版済み - 5 15 2001

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Phosphoglycerate Dehydrogenase
Serine
Astrocytes
Enzymes
Interleukin-6
Tumor Necrosis Factor-alpha
Messenger RNA
Frozen Sections
Microglia
Nitric Oxide Synthase Type II
Prosencephalon
Lipopolysaccharides
serine containing aminolipid
Nitric Oxide
Western Blotting
Staining and Labeling
Neurons
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

これを引用

L-serine regulates the activities of microglial cells that express very low level of 3-phosphoglycerate dehydrogenase, an enzyme for L-serine biosynthesis. / Sugishita, Hiroki; Kuwabara, Yasuhide; Toku, Kazuko; Doi, Lisa; Yang, Lihua; Mitoma, Junya; Furuya, Shigeki; Hirabayashi, Yoshio; Maeda, Nobuji; Sakanaka, Masahiro; Tanaka, Junya.

:: Journal of Neuroscience Research, 巻 64, 番号 4, 15.05.2001, p. 392-401.

研究成果: ジャーナルへの寄稿記事

Sugishita, H, Kuwabara, Y, Toku, K, Doi, L, Yang, L, Mitoma, J, Furuya, S, Hirabayashi, Y, Maeda, N, Sakanaka, M & Tanaka, J 2001, 'L-serine regulates the activities of microglial cells that express very low level of 3-phosphoglycerate dehydrogenase, an enzyme for L-serine biosynthesis', Journal of Neuroscience Research, 巻. 64, 番号 4, pp. 392-401. https://doi.org/10.1002/jnr.1090
Sugishita, Hiroki ; Kuwabara, Yasuhide ; Toku, Kazuko ; Doi, Lisa ; Yang, Lihua ; Mitoma, Junya ; Furuya, Shigeki ; Hirabayashi, Yoshio ; Maeda, Nobuji ; Sakanaka, Masahiro ; Tanaka, Junya. / L-serine regulates the activities of microglial cells that express very low level of 3-phosphoglycerate dehydrogenase, an enzyme for L-serine biosynthesis. :: Journal of Neuroscience Research. 2001 ; 巻 64, 番号 4. pp. 392-401.
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abstract = "Microglia are well known to become activated during various kinds of neuropathological events. The factors that are responsible for the activation, however, are not fully determined. In the present study, L-Ser was shown to enhance production of nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor α (TNF α) by lipopolysaccharide (LPS)-stimulated cultured rat microglial cells. L-Ser, however, did not enhance the expression of mRNAs encoding inducible NO synthase, IL-6 and TNF α. On the other hand, astrocytes did not depend on L-Ser for release of IL-6 and TNF α. The expression of an enzyme 3-phosphoglycerate dehydrogenase (3PGDH), which is essential for L-Ser biosynthesis from a glycolytic intermediate 3-phosphoglycerate, was investigated. As revealed by Western blotting and immunocytochemical staining, 3PGDH-protein expression in vitro was the highest in astrocytes, intermediate in neurons and the lowest in microglial cells. Semiquantitative RT-PCR showed that microglial cells expressed 3PGDH-mRNA at a lower level than astrocytes. In frozen sections from rat forebrain, only astrocytes were immunoreactive for 3PGDH. The present study suggested that L-Ser is able to modulate microglial function mainly at the translation level because microglial cells cannot synthesize sufficient amount of L-Ser due to the scarce expression of 3PGDH.",
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AU - Kuwabara, Yasuhide

AU - Toku, Kazuko

AU - Doi, Lisa

AU - Yang, Lihua

AU - Mitoma, Junya

AU - Furuya, Shigeki

AU - Hirabayashi, Yoshio

AU - Maeda, Nobuji

AU - Sakanaka, Masahiro

AU - Tanaka, Junya

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