Label-free enzymatic reaction monitoring in water-in-oil microdroplets using ultra-broadband multiplex coherent anti-Stokes Raman scattering spectroscopy

Ryo Imai, Hideaki Kano

研究成果: ジャーナルへの寄稿学術誌査読

抄録

We propose a system for monitoring an enzymatic reaction, i.e., dehydrogenation of ethanol catalyzed by alcohol dehydrogenase, in microdroplets using ultra-broadband multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopy. The reaction solution was encapsulated in water-in-oil microdroplets with diameters of 50 μm. The reaction was monitored by measuring the concentration of coenzymes from the CARS spectrum obtained in one-second exposure time. The results obtained using our system was consistent with those of the conventional fluorescence measurement system and indicate the potential of CARS spectroscopy for dropletbased high-throughput screening of enzymes.

本文言語英語
ページ(範囲)1506-1515
ページ数10
ジャーナルBiomedical Optics Express
13
3
DOI
出版ステータス出版済み - 3月 2022

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 原子分子物理学および光学

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