Label-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor

Ekkachai Kittiloespaisan, Akio Ojida, Itaru Hamachi, Yortyot Seetang-Nun, Wansika Kiatpathomchai, Jirarut Wongkongkatep

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

A label-free fluorescent detection method for the loopmediated isothermal amplification (LAMP) of nucleic acids was first developed by using the pyrophosphate-selective xanthenebased Zn(II)-coordination chemosensor 12Zn(II). In this method, the production of inorganic pyrophosphate (PPi), a by-product of the LAMP reaction, was detected by 12Zn(II) with a large fluorescence enhancement. On combination with the fluorescent plate reader technique, this method allows us to detect the shrimp white spot syndrome virus in a highthroughput manner with high sensitivity and selectivity.

元の言語英語
ページ(範囲)1666-1668
ページ数3
ジャーナルChemistry Letters
41
発行部数12
DOI
出版物ステータス出版済み - 2012

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Xanthenes
Nucleic Acids
Amplification
Labels
Viruses
Byproducts
Fluorescence
diphosphoric acid

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

これを引用

Label-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor. / Kittiloespaisan, Ekkachai; Ojida, Akio; Hamachi, Itaru; Seetang-Nun, Yortyot; Kiatpathomchai, Wansika; Wongkongkatep, Jirarut.

:: Chemistry Letters, 巻 41, 番号 12, 2012, p. 1666-1668.

研究成果: ジャーナルへの寄稿記事

Kittiloespaisan, Ekkachai ; Ojida, Akio ; Hamachi, Itaru ; Seetang-Nun, Yortyot ; Kiatpathomchai, Wansika ; Wongkongkatep, Jirarut. / Label-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor. :: Chemistry Letters. 2012 ; 巻 41, 番号 12. pp. 1666-1668.
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