Label-free imaging of acanthamoeba using multimodal nonlinear optical microscopy

Tsubasa Kobayashi, Yu Rok Cha, Yuichi Kaji, Tetsuro Oshika, Philippe Leproux, Vincent Couderc, Hideaki Kano

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

Acanthamoeba keratitis is a disease in which amoebae named Acanthamoeba invade the cornea of an eye. To diagnose this disease before it becomes serious, it is important to detect the cyst state of Acanthamoeba in the early stage of infection. In the present study, we explored spectroscopic signitures of the cyst state of Acanthamoeba using multimodal nonlinear optical microscopy with the channels of multiplex coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG), and third harmonic generation (THG). A sharp band at around 1603 cm-1 in the CARS (Im[χ(3)]) spectrum was found at the cyst state of Acanthamoeba, which possibly originates from ergosterol and/or 7-dehydrostigmasterol. It can be used as a maker band of Acanthamoeba for medical treatment. Keyword: Acanthamoeba keratitis, coherent anti-Stokes Raman scattering, CARS, second harmonic generation, SHG, microspectroscopy, multiphoton microscopy.

本文言語英語
ホスト出版物のタイトルMultiphoton Microscopy in the Biomedical Sciences XVIII
編集者Ammasi Periasamy, Xiaoliang S. Xie, Xiaoliang S. Xie, Karsten Konig, Peter T. C. So
出版社SPIE
ISBN(電子版)9781510614819
DOI
出版ステータス出版済み - 2018
外部発表はい
イベントMultiphoton Microscopy in the Biomedical Sciences XVIII 2018 - San Francisco, 米国
継続期間: 1 28 20181 30 2018

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
10498
ISSN(印刷版)1605-7422

会議

会議Multiphoton Microscopy in the Biomedical Sciences XVIII 2018
国/地域米国
CitySan Francisco
Period1/28/181/30/18

All Science Journal Classification (ASJC) codes

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 原子分子物理学および光学
  • 放射線学、核医学およびイメージング

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