Laxative Poisoning: Toxicological analysis of bisacodyl and its metabolite in urine, serum, and stool

Keiko Kudo, Chiaki Miyazaki, Ryo Kadoya, Tohru Imamura, Narumi Jitsufuchi, Noriaki Ikeda

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

An 11-year-old boy was hospitalized because of severe diarrhea (over 5 L/day). The child survived; however, the diarrhea continued while he was given intravenous fluids and his electrolyte balance was closely supervised. Based on observation of the patient and his family in the hospital, surreptitious administration of a poison by his mother was suspected, and toxicological analysis was carried out on stools from the boy and on medicine administered to him by his mother. Bisacodyl, a cathartic with a direct effect on the colon, was detected in the medicine, and a metabolite of bisacodyl was present in the stool. We devised a sensitive and reliable method to quantitate the bisacodyl metabolite in urine and serum. The sample was incubated with β-glucuronidase at 37°C for 2 h; bisacodyl metabolite was extracted with tert-butyl methyl ether, then derivatized by methylation, and subjected to gas chromatography-mass spectrometry. Bisphenol A was used as an internal standard. The calibration curve was linear in the concentration range from 2 to 1000 ng/0.2 mL, and the lower limits of detection were 1 ng/0.2 ml. for the urine and 2 ng/0.2 mL for the serum. As concentrations of bisacodyl metabolite in the urine and serum of the patient were clearly defined, perhaps such investigations are warranted before extensive clinical therapy is prescribed.

元の言語英語
ページ(範囲)274-278
ページ数5
ジャーナルJournal of Analytical Toxicology
22
発行部数4
DOI
出版物ステータス出版済み - 1 1 1998

Fingerprint

Bisacodyl
Laxatives
Metabolites
poisoning
urine
Toxicology
Poisoning
serum
metabolite
Urine
Serum
Water-Electrolyte Balance
medicine
Medicine
Diarrhea
Methylation
MTBE
Mothers
Cathartics
Hospital Administration

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

これを引用

Laxative Poisoning : Toxicological analysis of bisacodyl and its metabolite in urine, serum, and stool. / Kudo, Keiko; Miyazaki, Chiaki; Kadoya, Ryo; Imamura, Tohru; Jitsufuchi, Narumi; Ikeda, Noriaki.

:: Journal of Analytical Toxicology, 巻 22, 番号 4, 01.01.1998, p. 274-278.

研究成果: ジャーナルへの寄稿記事

Kudo, Keiko ; Miyazaki, Chiaki ; Kadoya, Ryo ; Imamura, Tohru ; Jitsufuchi, Narumi ; Ikeda, Noriaki. / Laxative Poisoning : Toxicological analysis of bisacodyl and its metabolite in urine, serum, and stool. :: Journal of Analytical Toxicology. 1998 ; 巻 22, 番号 4. pp. 274-278.
@article{a9d2649b75954535997479ca7559dd70,
title = "Laxative Poisoning: Toxicological analysis of bisacodyl and its metabolite in urine, serum, and stool",
abstract = "An 11-year-old boy was hospitalized because of severe diarrhea (over 5 L/day). The child survived; however, the diarrhea continued while he was given intravenous fluids and his electrolyte balance was closely supervised. Based on observation of the patient and his family in the hospital, surreptitious administration of a poison by his mother was suspected, and toxicological analysis was carried out on stools from the boy and on medicine administered to him by his mother. Bisacodyl, a cathartic with a direct effect on the colon, was detected in the medicine, and a metabolite of bisacodyl was present in the stool. We devised a sensitive and reliable method to quantitate the bisacodyl metabolite in urine and serum. The sample was incubated with β-glucuronidase at 37°C for 2 h; bisacodyl metabolite was extracted with tert-butyl methyl ether, then derivatized by methylation, and subjected to gas chromatography-mass spectrometry. Bisphenol A was used as an internal standard. The calibration curve was linear in the concentration range from 2 to 1000 ng/0.2 mL, and the lower limits of detection were 1 ng/0.2 ml. for the urine and 2 ng/0.2 mL for the serum. As concentrations of bisacodyl metabolite in the urine and serum of the patient were clearly defined, perhaps such investigations are warranted before extensive clinical therapy is prescribed.",
author = "Keiko Kudo and Chiaki Miyazaki and Ryo Kadoya and Tohru Imamura and Narumi Jitsufuchi and Noriaki Ikeda",
year = "1998",
month = "1",
day = "1",
doi = "10.1093/jat/22.4.274",
language = "English",
volume = "22",
pages = "274--278",
journal = "Journal of Analytical Toxicology",
issn = "0146-4760",
publisher = "Preston Publications",
number = "4",

}

TY - JOUR

T1 - Laxative Poisoning

T2 - Toxicological analysis of bisacodyl and its metabolite in urine, serum, and stool

AU - Kudo, Keiko

AU - Miyazaki, Chiaki

AU - Kadoya, Ryo

AU - Imamura, Tohru

AU - Jitsufuchi, Narumi

AU - Ikeda, Noriaki

PY - 1998/1/1

Y1 - 1998/1/1

N2 - An 11-year-old boy was hospitalized because of severe diarrhea (over 5 L/day). The child survived; however, the diarrhea continued while he was given intravenous fluids and his electrolyte balance was closely supervised. Based on observation of the patient and his family in the hospital, surreptitious administration of a poison by his mother was suspected, and toxicological analysis was carried out on stools from the boy and on medicine administered to him by his mother. Bisacodyl, a cathartic with a direct effect on the colon, was detected in the medicine, and a metabolite of bisacodyl was present in the stool. We devised a sensitive and reliable method to quantitate the bisacodyl metabolite in urine and serum. The sample was incubated with β-glucuronidase at 37°C for 2 h; bisacodyl metabolite was extracted with tert-butyl methyl ether, then derivatized by methylation, and subjected to gas chromatography-mass spectrometry. Bisphenol A was used as an internal standard. The calibration curve was linear in the concentration range from 2 to 1000 ng/0.2 mL, and the lower limits of detection were 1 ng/0.2 ml. for the urine and 2 ng/0.2 mL for the serum. As concentrations of bisacodyl metabolite in the urine and serum of the patient were clearly defined, perhaps such investigations are warranted before extensive clinical therapy is prescribed.

AB - An 11-year-old boy was hospitalized because of severe diarrhea (over 5 L/day). The child survived; however, the diarrhea continued while he was given intravenous fluids and his electrolyte balance was closely supervised. Based on observation of the patient and his family in the hospital, surreptitious administration of a poison by his mother was suspected, and toxicological analysis was carried out on stools from the boy and on medicine administered to him by his mother. Bisacodyl, a cathartic with a direct effect on the colon, was detected in the medicine, and a metabolite of bisacodyl was present in the stool. We devised a sensitive and reliable method to quantitate the bisacodyl metabolite in urine and serum. The sample was incubated with β-glucuronidase at 37°C for 2 h; bisacodyl metabolite was extracted with tert-butyl methyl ether, then derivatized by methylation, and subjected to gas chromatography-mass spectrometry. Bisphenol A was used as an internal standard. The calibration curve was linear in the concentration range from 2 to 1000 ng/0.2 mL, and the lower limits of detection were 1 ng/0.2 ml. for the urine and 2 ng/0.2 mL for the serum. As concentrations of bisacodyl metabolite in the urine and serum of the patient were clearly defined, perhaps such investigations are warranted before extensive clinical therapy is prescribed.

UR - http://www.scopus.com/inward/record.url?scp=0031877304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031877304&partnerID=8YFLogxK

U2 - 10.1093/jat/22.4.274

DO - 10.1093/jat/22.4.274

M3 - Article

C2 - 9681329

AN - SCOPUS:0031877304

VL - 22

SP - 274

EP - 278

JO - Journal of Analytical Toxicology

JF - Journal of Analytical Toxicology

SN - 0146-4760

IS - 4

ER -