抄録
An 11-year-old boy was hospitalized because of severe diarrhea (over 5 L/day). The child survived; however, the diarrhea continued while he was given intravenous fluids and his electrolyte balance was closely supervised. Based on observation of the patient and his family in the hospital, surreptitious administration of a poison by his mother was suspected, and toxicological analysis was carried out on stools from the boy and on medicine administered to him by his mother. Bisacodyl, a cathartic with a direct effect on the colon, was detected in the medicine, and a metabolite of bisacodyl was present in the stool. We devised a sensitive and reliable method to quantitate the bisacodyl metabolite in urine and serum. The sample was incubated with β-glucuronidase at 37°C for 2 h; bisacodyl metabolite was extracted with tert-butyl methyl ether, then derivatized by methylation, and subjected to gas chromatography-mass spectrometry. Bisphenol A was used as an internal standard. The calibration curve was linear in the concentration range from 2 to 1000 ng/0.2 mL, and the lower limits of detection were 1 ng/0.2 ml. for the urine and 2 ng/0.2 mL for the serum. As concentrations of bisacodyl metabolite in the urine and serum of the patient were clearly defined, perhaps such investigations are warranted before extensive clinical therapy is prescribed.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 274-278 |
| ページ数 | 5 |
| ジャーナル | Journal of Analytical Toxicology |
| 巻 | 22 |
| 発行部数 | 4 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 1998 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Environmental Chemistry
- Toxicology
- Health, Toxicology and Mutagenesis
- Chemical Health and Safety
これを引用
Laxative Poisoning : Toxicological analysis of bisacodyl and its metabolite in urine, serum, and stool. / Kudo, Keiko; Miyazaki, Chiaki; Kadoya, Ryo; Imamura, Tohru; Jitsufuchi, Narumi; Ikeda, Noriaki.
：: Journal of Analytical Toxicology, 巻 22, 番号 4, 01.01.1998, p. 274-278.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Laxative Poisoning
T2 - Toxicological analysis of bisacodyl and its metabolite in urine, serum, and stool
AU - Kudo, Keiko
AU - Miyazaki, Chiaki
AU - Kadoya, Ryo
AU - Imamura, Tohru
AU - Jitsufuchi, Narumi
AU - Ikeda, Noriaki
PY - 1998/1/1
Y1 - 1998/1/1
N2 - An 11-year-old boy was hospitalized because of severe diarrhea (over 5 L/day). The child survived; however, the diarrhea continued while he was given intravenous fluids and his electrolyte balance was closely supervised. Based on observation of the patient and his family in the hospital, surreptitious administration of a poison by his mother was suspected, and toxicological analysis was carried out on stools from the boy and on medicine administered to him by his mother. Bisacodyl, a cathartic with a direct effect on the colon, was detected in the medicine, and a metabolite of bisacodyl was present in the stool. We devised a sensitive and reliable method to quantitate the bisacodyl metabolite in urine and serum. The sample was incubated with β-glucuronidase at 37°C for 2 h; bisacodyl metabolite was extracted with tert-butyl methyl ether, then derivatized by methylation, and subjected to gas chromatography-mass spectrometry. Bisphenol A was used as an internal standard. The calibration curve was linear in the concentration range from 2 to 1000 ng/0.2 mL, and the lower limits of detection were 1 ng/0.2 ml. for the urine and 2 ng/0.2 mL for the serum. As concentrations of bisacodyl metabolite in the urine and serum of the patient were clearly defined, perhaps such investigations are warranted before extensive clinical therapy is prescribed.
AB - An 11-year-old boy was hospitalized because of severe diarrhea (over 5 L/day). The child survived; however, the diarrhea continued while he was given intravenous fluids and his electrolyte balance was closely supervised. Based on observation of the patient and his family in the hospital, surreptitious administration of a poison by his mother was suspected, and toxicological analysis was carried out on stools from the boy and on medicine administered to him by his mother. Bisacodyl, a cathartic with a direct effect on the colon, was detected in the medicine, and a metabolite of bisacodyl was present in the stool. We devised a sensitive and reliable method to quantitate the bisacodyl metabolite in urine and serum. The sample was incubated with β-glucuronidase at 37°C for 2 h; bisacodyl metabolite was extracted with tert-butyl methyl ether, then derivatized by methylation, and subjected to gas chromatography-mass spectrometry. Bisphenol A was used as an internal standard. The calibration curve was linear in the concentration range from 2 to 1000 ng/0.2 mL, and the lower limits of detection were 1 ng/0.2 ml. for the urine and 2 ng/0.2 mL for the serum. As concentrations of bisacodyl metabolite in the urine and serum of the patient were clearly defined, perhaps such investigations are warranted before extensive clinical therapy is prescribed.
UR - http://www.scopus.com/inward/record.url?scp=0031877304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031877304&partnerID=8YFLogxK
U2 - 10.1093/jat/22.4.274
DO - 10.1093/jat/22.4.274
M3 - Article
C2 - 9681329
AN - SCOPUS:0031877304
VL - 22
SP - 274
EP - 278
JO - Journal of Analytical Toxicology
JF - Journal of Analytical Toxicology
SN - 0146-4760
IS - 4
ER -