### 抄録

We have studied the dependence on microtubule length of sliding velocity and positional fluctuation from recorded trajectories of microtubules sliding over sea urchin sperm outer arm β dynein in a motility assay in vitro. The positional fluctuation was quantified by calculating the mean-square displacement deviation from the average, the calculation of which yields an effective diffusion coefficient. We have found that (1) the sliding velocity depends hyperbolically on the microtubule length, and (2) the effective diffusion coefficients do not depend on the length for sufficiently long microtubules. The length dependence of the sliding velocity indicates that the duty ratio, defined as the force producing period over the total cycle time of β dynein interaction with microtubule, is very small. The length independence of the effective diffusion coefficient indicates that there is a correlation in the sliding movement fluctuation of microtubules.

元の言語 | 英語 |
---|---|

ページ（範囲） | 117-125 |

ページ数 | 9 |

ジャーナル | Biophysical Chemistry |

巻 | 67 |

発行部数 | 1-3 |

DOI | |

出版物ステータス | 出版済み - 9 1 1997 |

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### All Science Journal Classification (ASJC) codes

- Biophysics
- Biochemistry
- Organic Chemistry

### これを引用

*Biophysical Chemistry*,

*67*(1-3), 117-125. https://doi.org/10.1016/S0301-4622(97)00028-8

**Length dependence of displacement fluctuations and velocity in microtubule sliding movement driven by sea urchin sperm outer arm β dynein in vitro.** / Imafuku, Yasuhiro; Toyoshima, Yoko Y.; Tawada, Katsuhisa.

研究成果: ジャーナルへの寄稿 › 記事

*Biophysical Chemistry*, 巻. 67, 番号 1-3, pp. 117-125. https://doi.org/10.1016/S0301-4622(97)00028-8

}

TY - JOUR

T1 - Length dependence of displacement fluctuations and velocity in microtubule sliding movement driven by sea urchin sperm outer arm β dynein in vitro

AU - Imafuku, Yasuhiro

AU - Toyoshima, Yoko Y.

AU - Tawada, Katsuhisa

PY - 1997/9/1

Y1 - 1997/9/1

N2 - We have studied the dependence on microtubule length of sliding velocity and positional fluctuation from recorded trajectories of microtubules sliding over sea urchin sperm outer arm β dynein in a motility assay in vitro. The positional fluctuation was quantified by calculating the mean-square displacement deviation from the average, the calculation of which yields an effective diffusion coefficient. We have found that (1) the sliding velocity depends hyperbolically on the microtubule length, and (2) the effective diffusion coefficients do not depend on the length for sufficiently long microtubules. The length dependence of the sliding velocity indicates that the duty ratio, defined as the force producing period over the total cycle time of β dynein interaction with microtubule, is very small. The length independence of the effective diffusion coefficient indicates that there is a correlation in the sliding movement fluctuation of microtubules.

AB - We have studied the dependence on microtubule length of sliding velocity and positional fluctuation from recorded trajectories of microtubules sliding over sea urchin sperm outer arm β dynein in a motility assay in vitro. The positional fluctuation was quantified by calculating the mean-square displacement deviation from the average, the calculation of which yields an effective diffusion coefficient. We have found that (1) the sliding velocity depends hyperbolically on the microtubule length, and (2) the effective diffusion coefficients do not depend on the length for sufficiently long microtubules. The length dependence of the sliding velocity indicates that the duty ratio, defined as the force producing period over the total cycle time of β dynein interaction with microtubule, is very small. The length independence of the effective diffusion coefficient indicates that there is a correlation in the sliding movement fluctuation of microtubules.

UR - http://www.scopus.com/inward/record.url?scp=0030775253&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030775253&partnerID=8YFLogxK

U2 - 10.1016/S0301-4622(97)00028-8

DO - 10.1016/S0301-4622(97)00028-8

M3 - Article

C2 - 17029893

AN - SCOPUS:0030775253

VL - 67

SP - 117

EP - 125

JO - Biophysical Chemistry

JF - Biophysical Chemistry

SN - 0301-4622

IS - 1-3

ER -