Length dependence of displacement fluctuations and velocity in microtubule sliding movement driven by sea urchin sperm outer arm β dynein in vitro

Yasuhiro Imafuku, Yoko Y. Toyoshima, Katsuhisa Tawada

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

We have studied the dependence on microtubule length of sliding velocity and positional fluctuation from recorded trajectories of microtubules sliding over sea urchin sperm outer arm β dynein in a motility assay in vitro. The positional fluctuation was quantified by calculating the mean-square displacement deviation from the average, the calculation of which yields an effective diffusion coefficient. We have found that (1) the sliding velocity depends hyperbolically on the microtubule length, and (2) the effective diffusion coefficients do not depend on the length for sufficiently long microtubules. The length dependence of the sliding velocity indicates that the duty ratio, defined as the force producing period over the total cycle time of β dynein interaction with microtubule, is very small. The length independence of the effective diffusion coefficient indicates that there is a correlation in the sliding movement fluctuation of microtubules.

元の言語英語
ページ(範囲)117-125
ページ数9
ジャーナルBiophysical Chemistry
67
発行部数1-3
DOI
出版物ステータス出版済み - 9 1 1997

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Dyneins
Sea Urchins
Microtubules
Spermatozoa
Assays
Trajectories
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Organic Chemistry

これを引用

Length dependence of displacement fluctuations and velocity in microtubule sliding movement driven by sea urchin sperm outer arm β dynein in vitro. / Imafuku, Yasuhiro; Toyoshima, Yoko Y.; Tawada, Katsuhisa.

:: Biophysical Chemistry, 巻 67, 番号 1-3, 01.09.1997, p. 117-125.

研究成果: ジャーナルへの寄稿記事

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