Lethal hydroxyl radical accumulation by a lactococcal bacteriocin, lacticin q

Mengqi Li, Fuminori Yoneyama, Nayu Toshimitsu, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto

研究成果: Contribution to journalArticle査読

14 被引用数 (Scopus)

抄録

The antimicrobial mechanism of a lactococcal bacteriocin, lacticin Q, can be described by the toroidal pore model without any receptor. However, lacticin Q showed different degrees of activity (selective antimicrobial activity) against Gram-positive bacteria even among related species. The ability of lacticin Q to induce pore formation in liposomes composed of lipids from different indicator strains indicated that its selective antimicrobial activity could not be attributed only to membrane lipid composition. We investigated the accumulation of deleterious hydroxyl radicals after exposure to lacticin Q as a contributing factor to cell death in the indicator strains. When lacticin Q of the same concentration as the MIC or minimum bactericidal concentration was added to the indicator cultures, high levels of hydroxyl radical accumulation were detected. Treatment with hydroxyl radical scavengers, thiourea and 2,2=-bipyridyl, decreased the levels of hydroxyl radical accumulation and recovered cell viability. These results suggest that, with or without pore formation, the final antimicrobial mechanism of lacticin Q is the accumulation of hydroxyl radicals, which varies by strain, resulting in the selective antimicrobial activity of lacticin Q.

本文言語英語
ページ(範囲)3897-3902
ページ数6
ジャーナルAntimicrobial Agents and Chemotherapy
57
8
DOI
出版ステータス出版済み - 8 2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 薬理学
  • 薬理学(医学)
  • 感染症

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