Leukotriene B4 augments and restores FcγRs-dependent phagocytosis in macrophages

Fuyuki Okamoto, Kazuko Saeki, Hideki Sumimoto, Shou Yamasaki, Takehiko Yokomizo

研究成果: ジャーナルへの寄稿記事

28 引用 (Scopus)

抄録

Phagocytosis by macrophages is essential for host defense, i.e. preventing invasion of pathogens and foreign materials. Macrophages engulf immunoglobulin G (IgG)-opsonized particles through the action of the receptors for the Fc of IgG (FcγRs). Leukotriene B4 (LTB4) is a classical lipid chemoattractant derived from arachidonic acid. Leukotriene B4 receptor 1 (BLT1), a high affinity LTB4 receptor, is expressed in a variety of immune cells such as neutrophils, macrophages, and dendritic cells. Although LTB4 has been shown to enhance macrophage phagocytosis, few studies have investigated the intracellular mechanisms involved in this in detail. Furthermore, there have been no reports of the direct cross-talk between LTB4-BLT1 and IgG-FcγRs signaling. Here, we show that FcγRs-dependent phagocytosis was attenuated in BLT1-deficient macrophages as compared with wild-type (WT) cells. Moreover, cross-talk between LTB 4-BLT1 and IgG-FcγRs signaling was identified at the level of phosphatidylinositol 3-OH kinase (PI3K) and Rac, downstream of Syk. In addition, the trimeric Gi protein (Gi) was found to be essential for BLT1-dependent phagocytosis. Surprisingly, we found that LTB 4-BLT1 signaling restores phagocytosis in the absence of FcγRs signaling. These data indicate that LTB4-BLT1 signaling plays a pivotal role in macrophage phagocytosis and innate immunity.

元の言語英語
ページ(範囲)41113-41121
ページ数9
ジャーナルJournal of Biological Chemistry
285
発行部数52
DOI
出版物ステータス出版済み - 12 24 2010

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Leukotriene B4
Macrophages
Phagocytosis
Leukotriene B4 Receptors
Immunoglobulin G
Immunoglobulin Fc Fragments
Chemotactic Factors
Pathogens
Phosphatidylinositol 3-Kinases
Innate Immunity
Arachidonic Acid
Dendritic Cells
Neutrophils
Lipids
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Leukotriene B4 augments and restores FcγRs-dependent phagocytosis in macrophages. / Okamoto, Fuyuki; Saeki, Kazuko; Sumimoto, Hideki; Yamasaki, Shou; Yokomizo, Takehiko.

:: Journal of Biological Chemistry, 巻 285, 番号 52, 24.12.2010, p. 41113-41121.

研究成果: ジャーナルへの寄稿記事

Okamoto, Fuyuki ; Saeki, Kazuko ; Sumimoto, Hideki ; Yamasaki, Shou ; Yokomizo, Takehiko. / Leukotriene B4 augments and restores FcγRs-dependent phagocytosis in macrophages. :: Journal of Biological Chemistry. 2010 ; 巻 285, 番号 52. pp. 41113-41121.
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