Ligand-receptor interaction-induced self-assembled architectures of proteins

Characterization of the interaction forces and manner of assembly NanoLEGO proteins

Tei Maki, Kengo Usui, Satoru Kidoaki, Harukazu Suzuki, Masayoshi Ito, Fuyu Ito, Takehisa Matsuda, Yoshihide Hayashizaki

研究成果: 会議への寄与タイプ論文

抄録

We have constructed the fusion proteins which contain multiple different specific binding sites and self-assemble to an ordered structure by their mutual specific interactions (termed NanoLEGO). Subunit of tetramer protein, super oxide reductase (SOR), was fused with PDZ protein, tax-interacting protein-1(TIP-1), and its recognition peptides(PDZ-pep). The genetically-modified subunits of SOR with TIP-1 and PDZ-pep could self-assemble and reconstruct the chimera tetramer with four binding sites. To investigate the self-assembling ability of SOR NanoLEGO, the interaction forces between TIP-1 and PDZ-pep were characterized by dynamic force spectroscopy, and the assembling property and structures were analyzed with turbidity measurement, phase contrast and fluorescence microscopy, and transmission electron microscopy. SOR NanoLEGOs were found to self-assemble and form chain-like constructs.

元の言語英語
ページ5284-5285
ページ数2
出版物ステータス出版済み - 12 1 2006
イベント55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, 日本
継続期間: 9 20 20069 22 2006

その他

その他55th Society of Polymer Science Japan Symposium on Macromolecules
日本
Toyama
期間9/20/069/22/06

Fingerprint

Ligands
Proteins
Taxation
Oxides
Binding sites
Phase measurement
Fluorescence microscopy
Turbidity
Peptides
Fusion reactions
Spectroscopy
Transmission electron microscopy
Oxidoreductases

All Science Journal Classification (ASJC) codes

  • Engineering(all)

これを引用

Maki, T., Usui, K., Kidoaki, S., Suzuki, H., Ito, M., Ito, F., ... Hayashizaki, Y. (2006). Ligand-receptor interaction-induced self-assembled architectures of proteins: Characterization of the interaction forces and manner of assembly NanoLEGO proteins. 5284-5285. 論文発表場所 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, 日本.

Ligand-receptor interaction-induced self-assembled architectures of proteins : Characterization of the interaction forces and manner of assembly NanoLEGO proteins. / Maki, Tei; Usui, Kengo; Kidoaki, Satoru; Suzuki, Harukazu; Ito, Masayoshi; Ito, Fuyu; Matsuda, Takehisa; Hayashizaki, Yoshihide.

2006. 5284-5285 論文発表場所 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, 日本.

研究成果: 会議への寄与タイプ論文

Maki, T, Usui, K, Kidoaki, S, Suzuki, H, Ito, M, Ito, F, Matsuda, T & Hayashizaki, Y 2006, 'Ligand-receptor interaction-induced self-assembled architectures of proteins: Characterization of the interaction forces and manner of assembly NanoLEGO proteins' 論文発表場所 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, 日本, 9/20/06 - 9/22/06, pp. 5284-5285.
Maki T, Usui K, Kidoaki S, Suzuki H, Ito M, Ito F その他. Ligand-receptor interaction-induced self-assembled architectures of proteins: Characterization of the interaction forces and manner of assembly NanoLEGO proteins. 2006. 論文発表場所 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, 日本.
Maki, Tei ; Usui, Kengo ; Kidoaki, Satoru ; Suzuki, Harukazu ; Ito, Masayoshi ; Ito, Fuyu ; Matsuda, Takehisa ; Hayashizaki, Yoshihide. / Ligand-receptor interaction-induced self-assembled architectures of proteins : Characterization of the interaction forces and manner of assembly NanoLEGO proteins. 論文発表場所 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, 日本.2 p.
@conference{db4141c94ca24a3ab950bc4b11000859,
title = "Ligand-receptor interaction-induced self-assembled architectures of proteins: Characterization of the interaction forces and manner of assembly NanoLEGO proteins",
abstract = "We have constructed the fusion proteins which contain multiple different specific binding sites and self-assemble to an ordered structure by their mutual specific interactions (termed NanoLEGO). Subunit of tetramer protein, super oxide reductase (SOR), was fused with PDZ protein, tax-interacting protein-1(TIP-1), and its recognition peptides(PDZ-pep). The genetically-modified subunits of SOR with TIP-1 and PDZ-pep could self-assemble and reconstruct the chimera tetramer with four binding sites. To investigate the self-assembling ability of SOR NanoLEGO, the interaction forces between TIP-1 and PDZ-pep were characterized by dynamic force spectroscopy, and the assembling property and structures were analyzed with turbidity measurement, phase contrast and fluorescence microscopy, and transmission electron microscopy. SOR NanoLEGOs were found to self-assemble and form chain-like constructs.",
author = "Tei Maki and Kengo Usui and Satoru Kidoaki and Harukazu Suzuki and Masayoshi Ito and Fuyu Ito and Takehisa Matsuda and Yoshihide Hayashizaki",
year = "2006",
month = "12",
day = "1",
language = "English",
pages = "5284--5285",
note = "55th Society of Polymer Science Japan Symposium on Macromolecules ; Conference date: 20-09-2006 Through 22-09-2006",

}

TY - CONF

T1 - Ligand-receptor interaction-induced self-assembled architectures of proteins

T2 - Characterization of the interaction forces and manner of assembly NanoLEGO proteins

AU - Maki, Tei

AU - Usui, Kengo

AU - Kidoaki, Satoru

AU - Suzuki, Harukazu

AU - Ito, Masayoshi

AU - Ito, Fuyu

AU - Matsuda, Takehisa

AU - Hayashizaki, Yoshihide

PY - 2006/12/1

Y1 - 2006/12/1

N2 - We have constructed the fusion proteins which contain multiple different specific binding sites and self-assemble to an ordered structure by their mutual specific interactions (termed NanoLEGO). Subunit of tetramer protein, super oxide reductase (SOR), was fused with PDZ protein, tax-interacting protein-1(TIP-1), and its recognition peptides(PDZ-pep). The genetically-modified subunits of SOR with TIP-1 and PDZ-pep could self-assemble and reconstruct the chimera tetramer with four binding sites. To investigate the self-assembling ability of SOR NanoLEGO, the interaction forces between TIP-1 and PDZ-pep were characterized by dynamic force spectroscopy, and the assembling property and structures were analyzed with turbidity measurement, phase contrast and fluorescence microscopy, and transmission electron microscopy. SOR NanoLEGOs were found to self-assemble and form chain-like constructs.

AB - We have constructed the fusion proteins which contain multiple different specific binding sites and self-assemble to an ordered structure by their mutual specific interactions (termed NanoLEGO). Subunit of tetramer protein, super oxide reductase (SOR), was fused with PDZ protein, tax-interacting protein-1(TIP-1), and its recognition peptides(PDZ-pep). The genetically-modified subunits of SOR with TIP-1 and PDZ-pep could self-assemble and reconstruct the chimera tetramer with four binding sites. To investigate the self-assembling ability of SOR NanoLEGO, the interaction forces between TIP-1 and PDZ-pep were characterized by dynamic force spectroscopy, and the assembling property and structures were analyzed with turbidity measurement, phase contrast and fluorescence microscopy, and transmission electron microscopy. SOR NanoLEGOs were found to self-assemble and form chain-like constructs.

UR - http://www.scopus.com/inward/record.url?scp=33846163802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846163802&partnerID=8YFLogxK

M3 - Paper

SP - 5284

EP - 5285

ER -