Ligand-receptor interaction-induced self-assembled architectures of proteins: Characterization of the interaction forces and manner of assembly NanoLEGO proteins

Tei Maki, Kengo Usui, Satoru Kidoaki, Harukazu Suzuki, Masayoshi Ito, Fuyu Ito, Takehisa Matsuda, Yoshihide Hayashizaki

研究成果: 会議への寄与タイプ学会誌査読

抄録

We have constructed the fusion proteins which contain multiple different specific binding sites and self-assemble to an ordered structure by their mutual specific interactions (termed NanoLEGO). Subunit of tetramer protein, super oxide reductase (SOR), was fused with PDZ protein, tax-interacting protein-1(TIP-1), and its recognition peptides(PDZ-pep). The genetically-modified subunits of SOR with TIP-1 and PDZ-pep could self-assemble and reconstruct the chimera tetramer with four binding sites. To investigate the self-assembling ability of SOR NanoLEGO, the interaction forces between TIP-1 and PDZ-pep were characterized by dynamic force spectroscopy, and the assembling property and structures were analyzed with turbidity measurement, phase contrast and fluorescence microscopy, and transmission electron microscopy. SOR NanoLEGOs were found to self-assemble and form chain-like constructs.

本文言語英語
ページ5284-5285
ページ数2
出版ステータス出版済み - 12月 1 2006
イベント55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, 日本
継続期間: 9月 20 20069月 22 2006

その他

その他55th Society of Polymer Science Japan Symposium on Macromolecules
国/地域日本
CityToyama
Period9/20/069/22/06

!!!All Science Journal Classification (ASJC) codes

  • 工学(全般)

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