Light chain 3 associates with a Sos1 guanine nucleotide exchange factor: Its significance in the Sos1-mediated Rac1 signaling leading to membrane ruffling

Shuichi Furuta, Koichi Miura, Terry Copeland, Wei Hao Shang, Aki Oshima, Tohru Kamata

研究成果: ジャーナルへの寄稿学術誌査読

14 被引用数 (Scopus)

抄録

A 19 kDa protein was identified to associate with the Dbl oncogene homology domain of Sos1 (Sos-DH) and was purified from rat brains by GST-Sos-DH affinity chromatography. Peptide sequencing revealed that the protein is identical to light chain 3 (LC3), a microtubule-associated protein. LC3 coimmunoprecipitated with Sos1, and GST-LC3 was capable of forming complexes with Sos1 in in vitro GST-pull down assay. Furthermore, LC3 was colocalized with Sos1 in cells, as determined by immunohistochemistry. While Sos1 stimulated the guanine nucleotide exchange reaction on Rac1, LC3 suppressed the ability of Sos1 to activate Rac1 in in vitro experiments using COS cell lysates. Consistent with this, overexpression of LC3 decreased the level of active GTP-bound Rac1 in COS cells. Sos1 expression induced membrane ruffling, a downstream target for Rac1, but LC3 expression inhibited this biological effect of Sos1. These findings suggest that LC3 interacts with Sos1 and thereby negatively regulates the Sos1-dependent Rac1 activation leading to membrane ruffling.

本文言語英語
ページ(範囲)7060-7066
ページ数7
ジャーナルOncogene
21
46
DOI
出版ステータス出版済み - 2002
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学
  • 癌研究

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