Light Controls Protein Localization through Phytochrome-Mediated Alternative Promoter Selection

Tomokazu Ushijima, Kousuke Hanada, Eiji Gotoh, Wataru Yamori, Yutaka Kodama, Hiroyuki Tanaka, Miyako Kusano, Atsushi Fukushima, Mutsutomo Tokizawa, Yoshiharu Y. Yamamoto, Yasuomi Tada, Yutaka Suzuki, Tomonao Matsushita

研究成果: ジャーナルへの寄稿記事

  • 7 引用

抄録

Alternative promoter usage is a proteome-expanding mechanism that allows multiple pre-mRNAs to be transcribed from a single gene. The impact of this mechanism on the proteome and whether it is positively exploited in normal organismal responses remain unclear. We found that the plant photoreceptor phytochrome induces genome-wide changes in alternative promoter selection in Arabidopsis thaliana. Through this mechanism, protein isoforms with different N termini are produced that display light-dependent differences in localization. For instance, shade-grown plants accumulate a cytoplasmic isoform of glycerate kinase (GLYK), an essential photorespiration enzyme that was previously thought to localize exclusively to the chloroplast. Cytoplasmic GLYK constitutes a photorespiratory bypass that alleviates fluctuating light-induced photoinhibition. Therefore, phytochrome controls alternative promoter selection to modulate protein localization in response to changing light conditions. This study suggests that alternative promoter usage represents another ubiquitous layer of gene expression regulation in eukaryotes that contributes to diversification of the proteome. Light signaling through phytochrome receptors changes protein localization through alternative promoter selection, allowing plants to metabolically respond to changing light conditions.

言語英語
ページ1316-1325.e12
ジャーナルCell
Volume171
6
DOIs
State出版済み - 11 30 2017

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Phytochrome
glycerate kinase
Light
Proteome
Proteins
Plant Photoreceptors
Gene expression regulation
Protein Isoforms
Genes
RNA Precursors
Gene Expression Regulation
Chloroplasts
Eukaryota
Arabidopsis
Genome
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Light Controls Protein Localization through Phytochrome-Mediated Alternative Promoter Selection. / Ushijima, Tomokazu; Hanada, Kousuke; Gotoh, Eiji; Yamori, Wataru; Kodama, Yutaka; Tanaka, Hiroyuki; Kusano, Miyako; Fukushima, Atsushi; Tokizawa, Mutsutomo; Yamamoto, Yoshiharu Y.; Tada, Yasuomi; Suzuki, Yutaka; Matsushita, Tomonao.

:: Cell, 巻 171, 番号 6, 30.11.2017, p. 1316-1325.e12.

研究成果: ジャーナルへの寄稿記事

Ushijima, T, Hanada, K, Gotoh, E, Yamori, W, Kodama, Y, Tanaka, H, Kusano, M, Fukushima, A, Tokizawa, M, Yamamoto, YY, Tada, Y, Suzuki, Y & Matsushita, T 2017, 'Light Controls Protein Localization through Phytochrome-Mediated Alternative Promoter Selection' Cell, 巻. 171, 番号 6, pp. 1316-1325.e12. DOI: 10.1016/j.cell.2017.10.018
Ushijima T, Hanada K, Gotoh E, Yamori W, Kodama Y, Tanaka H その他. Light Controls Protein Localization through Phytochrome-Mediated Alternative Promoter Selection. Cell. 2017 11 30;171(6):1316-1325.e12. 利用可能場所, DOI: 10.1016/j.cell.2017.10.018
Ushijima, Tomokazu ; Hanada, Kousuke ; Gotoh, Eiji ; Yamori, Wataru ; Kodama, Yutaka ; Tanaka, Hiroyuki ; Kusano, Miyako ; Fukushima, Atsushi ; Tokizawa, Mutsutomo ; Yamamoto, Yoshiharu Y. ; Tada, Yasuomi ; Suzuki, Yutaka ; Matsushita, Tomonao. / Light Controls Protein Localization through Phytochrome-Mediated Alternative Promoter Selection. :: Cell. 2017 ; 巻 171, 番号 6. pp. 1316-1325.e12
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