Light-sheet microscopy-based 3D single-cell tracking reveals a correlation between cell cycle and the start of endoderm cell internalization in early zebrafish development

Akiko Kondow, Kiyoshi Ohnuma, Yasuhiro Kamei, Atsushi Taniguchi, Ryoma Bise, Yoichi Sato, Hisateru Yamaguchi, Shigenori Nonaka, Keiichiro Hashimoto

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

Controlling the initiation of cell migration plays a fundamental role in shaping the tissue during embryonic development. During gastrulation in zebrafish, some mesendoderm cells migrate inward to form the endoderm as the innermost germ layer along the yolk syncytial layer. However, how the initiation of inward migration is regulated is poorly understood. In this study, we performed light-sheet microscopy-based 3D single-cell tracking consisting of (a) whole-embryo time-lapse imaging with light-sheet microscopy and (b) three-dimensional single cell tracking in the zebrafish gastrula in which cells are marked with histone H2A-mCherry (nuclei) and the sox17:EGFP transgene (expressed in endoderm cells). We analyzed the correlation between the timing of cell internalization and cell division. Most cells that differentiated into endoderm cells began to internalize during the first half of the cell cycle, where the length of a cell cycle was defined by the period between two successive cell divisions. By contrast, the timing of other internalized cells was not correlated with a certain phase of the cell cycle. These results suggest the possibility that cell differentiation is associated with the relationship between cell cycle progression and the start of internalization. Moreover, the 3D single-cell tracking approach is useful for further investigating how cell migration is integrated with cell proliferation to shape tissues in zebrafish embryos.

本文言語英語
ページ(範囲)495-502
ページ数8
ジャーナルDevelopment Growth and Differentiation
62
7-8
DOI
出版ステータス出版済み - 10 1 2020

All Science Journal Classification (ASJC) codes

  • 発生生物学
  • 細胞生物学

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