Through carrying out Raman spectroscopy under a microscope, we can observe the molecular distribution and dynamics of living cells in situ without staining or labeling. In general, Raman microscopy/microspectroscopy is classified into spontaneous Raman microscopy/microspectroscopy and nonlinear Raman microscopy/microspectroscopy. This review will first summarize the historical development of Raman microscopes, and will discuss in detail their instrumentation and applications.
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics