Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element

Takahiro Kusakabe, Yasushi Sugimoto, Takuji Maeda, Yumiko Nakajima, Masaru Miyano, Jun Ichi Nishikawa, Shigenobu Tone, Yutaka Kawaguchi, Katsumi Koga, Takashi Ohyama

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

A bent DNA library was constructed from human genomic DNA, from which a new clone belonging to the human LINE-1 sequence family was isolated and characterized. This clone, with a length of 378 base pairs and termed HBC-1 (human bent clone-1), contained an intrinsically occurring curved DNA structure. By permutation analysis, the center of curvature of this fragment was mapped onto the nucleotide position 886 from the 5′ terminus of the complete LINE-1 sequence. Reporter plasmids, which contain HBC-1, were effectively integrated into human chromosome, indicating that the bent DNA structure provides a preferential donor site for the integration of human LINE-1 sequences. The present finding may provide an explanation as to why some inactivated LINE-1 sequences on human chromosomes carry the deletion at their 5′ termini.

元の言語英語
ページ(範囲)271-281
ページ数11
ジャーナルGene
274
発行部数1-2
DOI
出版物ステータス出版済み - 8 22 2001

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Long Interspersed Nucleotide Elements
Clone Cells
DNA
Human Chromosomes
Chromosome Deletion
Gene Library
Base Pairing
Plasmids
Nucleotides

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element. / Kusakabe, Takahiro; Sugimoto, Yasushi; Maeda, Takuji; Nakajima, Yumiko; Miyano, Masaru; Nishikawa, Jun Ichi; Tone, Shigenobu; Kawaguchi, Yutaka; Koga, Katsumi; Ohyama, Takashi.

:: Gene, 巻 274, 番号 1-2, 22.08.2001, p. 271-281.

研究成果: ジャーナルへの寄稿記事

Kusakabe, T, Sugimoto, Y, Maeda, T, Nakajima, Y, Miyano, M, Nishikawa, JI, Tone, S, Kawaguchi, Y, Koga, K & Ohyama, T 2001, 'Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element', Gene, 巻. 274, 番号 1-2, pp. 271-281. https://doi.org/10.1016/S0378-1119(01)00631-X
Kusakabe, Takahiro ; Sugimoto, Yasushi ; Maeda, Takuji ; Nakajima, Yumiko ; Miyano, Masaru ; Nishikawa, Jun Ichi ; Tone, Shigenobu ; Kawaguchi, Yutaka ; Koga, Katsumi ; Ohyama, Takashi. / Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element. :: Gene. 2001 ; 巻 274, 番号 1-2. pp. 271-281.
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abstract = "A bent DNA library was constructed from human genomic DNA, from which a new clone belonging to the human LINE-1 sequence family was isolated and characterized. This clone, with a length of 378 base pairs and termed HBC-1 (human bent clone-1), contained an intrinsically occurring curved DNA structure. By permutation analysis, the center of curvature of this fragment was mapped onto the nucleotide position 886 from the 5′ terminus of the complete LINE-1 sequence. Reporter plasmids, which contain HBC-1, were effectively integrated into human chromosome, indicating that the bent DNA structure provides a preferential donor site for the integration of human LINE-1 sequences. The present finding may provide an explanation as to why some inactivated LINE-1 sequences on human chromosomes carry the deletion at their 5′ termini.",
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AU - Sugimoto, Yasushi

AU - Maeda, Takuji

AU - Nakajima, Yumiko

AU - Miyano, Masaru

AU - Nishikawa, Jun Ichi

AU - Tone, Shigenobu

AU - Kawaguchi, Yutaka

AU - Koga, Katsumi

AU - Ohyama, Takashi

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