Lipase catalysis on monolayers at the air/water interface. 1. Kinetic rate constants on quasi-two-dimension

Keiji Tanaka, Patricia A. Manning, Hyuk Yu

研究成果: ジャーナルへの寄稿記事

18 引用 (Scopus)

抄録

Chemical reactions on a cell membrane surface are of pivotal importance for cellular functions such as intracellular signal transduction. Of the variety of membrane proteins, those imbedded in hemi-leaflets of bilayer membranes can be mimicked structurally and dynamically on monomolecular layers of phospholipids at the air/water interface. This is to report the kinetics of lipase (Pseudomonas cepacia) catalyzed hydrolysis of a substrate (umbelliferone stearate) on uniphasic L-α-dilauroylphosphatidylcholine monolayers at the air/water interface. A novel experimental protocol of the interfacial reaction is employed to probe the enzymatic kinetics. The kinetic rate constants on the monolayers are extracted from the substrate and the enzyme concentration dependences of the initial hydrolysis rate. The lipase on monolayers at the air/water interface is highly activated over that in bulk solution, and the turnover number of the lipase catalysis is strongly dependent on the surface pressure of monolayers.

元の言語英語
ページ(範囲)2665-2671
ページ数7
ジャーナルLangmuir
16
発行部数6
DOI
出版物ステータス出版済み - 3 21 2000

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Lipases
Lipase
Catalysis
catalysis
Monolayers
Rate constants
Kinetics
hydrolysis
Water
air
kinetics
Air
7-hydroxycoumarin
water
membranes
stearates
pseudomonas
Hydrolysis
enzymes
chemical reactions

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

これを引用

Lipase catalysis on monolayers at the air/water interface. 1. Kinetic rate constants on quasi-two-dimension. / Tanaka, Keiji; Manning, Patricia A.; Yu, Hyuk.

:: Langmuir, 巻 16, 番号 6, 21.03.2000, p. 2665-2671.

研究成果: ジャーナルへの寄稿記事

Tanaka, Keiji ; Manning, Patricia A. ; Yu, Hyuk. / Lipase catalysis on monolayers at the air/water interface. 1. Kinetic rate constants on quasi-two-dimension. :: Langmuir. 2000 ; 巻 16, 番号 6. pp. 2665-2671.
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