Lipase catalysis on monolayers at the air/water interface. 2. Diffusion-controlled kinetics on quasi-two-dimension

Keiji Tanaka, Steven P. Mecca, Hyuk Yu

研究成果: ジャーナルへの寄稿学術誌査読

8 被引用数 (Scopus)

抄録

Homogeneous exoplasmic leaflets of biomembrane are mimicked by lipid monolayers at the air/water interface. The reaction rates of lipase (Pseudomonas cepacia) catalyzed hydrolysis of a substrate (umbelliferone stearate) on L-α-dilauroylphosphatidylcholine/cholesterol mixed monolayers at the air/water interface are examined as a function of cholesterol composition, which is to vary the dynamics of the system. Lateral mobility as a measure of dynamics of phospholipids and adsorbed lipase molecules are probed with the technique of fluorescence recovery after photobleaching. Upon correlating the lateral diffusion coefficients of a probe lipid and lipase with the interfacial hydrolysis kinetics, we show for the first time that the catalytic reactions on the monolayers are diffusion-controlled. Moreover, our results are in a quantitative agreement with the two-dimensional reaction dynamics theory of Torney and McConnell.

本文言語英語
ページ(範囲)2672-2676
ページ数5
ジャーナルLangmuir
16
6
DOI
出版ステータス出版済み - 3月 21 2000
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 材料科学(全般)
  • 凝縮系物理学
  • 表面および界面
  • 分光学
  • 電気化学

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