Lipid Lateral Diffusion in Dilauroylphosphatidylcholine/Cholesterol Mixed Monolavers at the Air/Water Interface

Keiji Tanaka, Patricia A. Manning, Victor K. Lau, Hyuk Yu

研究成果: Contribution to journalArticle査読

37 被引用数 (Scopus)

抄録

Binary monolayers of L-α-dilauroylphosphatidylcholine (DLPC) and cholesterol were formed at the air/water interface by the successive addition method with chloroform as the spreading solvent at 296 K. Translational diffusion of a probe lipid in the monolayers as a function of cholesterol fraction was examined by the technique of fluorescence recovery after photobleaching in conjunction with epifluorescence microscopy and the Wilhelmy plate method. A transition from biphasic morphology to a visually homogeneous uniphasic monolayer occurred at surface pressures between 13 and 20 mN·m-1, depending on the cholesterol fraction in the monolayer. The lateral diffusion coefficient was found to decrease when surface pressure and/or cholesterol fraction were/was increased. The diffusion coefficients of the probe lipid in the homogeneous monolayers were analyzed on the basis of the free area model. The observed retardation of the diffusion is ascribed to an increase in the in-plane surface viscosity of monolayers. In the case of biphasic binary monolayers, the retardation with increasing cholesterol fraction is attributed to the obstacle mediated diffusion as in the effective medium analysis as well as to increasing surface viscosity.

本文言語英語
ページ(範囲)600-606
ページ数7
ジャーナルLangmuir
15
2
DOI
出版ステータス出版済み - 1 1 1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • 材料科学(全般)
  • 凝縮系物理学
  • 表面および界面
  • 分光学
  • 電気化学

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