Lipid Lateral Diffusion in Dilauroylphosphatidylcholine/Cholesterol Mixed Monolavers at the Air/Water Interface

Keiji Tanaka, Patricia A. Manning, Victor K. Lau, Hyuk Yu

研究成果: ジャーナルへの寄稿記事

36 引用 (Scopus)

抄録

Binary monolayers of L-α-dilauroylphosphatidylcholine (DLPC) and cholesterol were formed at the air/water interface by the successive addition method with chloroform as the spreading solvent at 296 K. Translational diffusion of a probe lipid in the monolayers as a function of cholesterol fraction was examined by the technique of fluorescence recovery after photobleaching in conjunction with epifluorescence microscopy and the Wilhelmy plate method. A transition from biphasic morphology to a visually homogeneous uniphasic monolayer occurred at surface pressures between 13 and 20 mN·m -1, depending on the cholesterol fraction in the monolayer. The lateral diffusion coefficient was found to decrease when surface pressure and/or cholesterol fraction were/was increased. The diffusion coefficients of the probe lipid in the homogeneous monolayers were analyzed on the basis of the free area model. The observed retardation of the diffusion is ascribed to an increase in the in-plane surface viscosity of monolayers. In the case of biphasic binary monolayers, the retardation with increasing cholesterol fraction is attributed to the obstacle mediated diffusion as in the effective medium analysis as well as to increasing surface viscosity.

元の言語英語
ページ(範囲)600-606
ページ数7
ジャーナルLangmuir
15
発行部数2
出版物ステータス出版済み - 12 1 1999

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Cholesterol
cholesterol
Lipids
lipids
Monolayers
Water
air
Air
water
diffusion coefficient
viscosity
probes
Viscosity
chloroform
Photobleaching
recovery
Chloroform
Chlorine compounds
1,2-dilauroylphosphatidylcholine
microscopy

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

これを引用

Lipid Lateral Diffusion in Dilauroylphosphatidylcholine/Cholesterol Mixed Monolavers at the Air/Water Interface. / Tanaka, Keiji; Manning, Patricia A.; Lau, Victor K.; Yu, Hyuk.

:: Langmuir, 巻 15, 番号 2, 01.12.1999, p. 600-606.

研究成果: ジャーナルへの寄稿記事

Tanaka, Keiji ; Manning, Patricia A. ; Lau, Victor K. ; Yu, Hyuk. / Lipid Lateral Diffusion in Dilauroylphosphatidylcholine/Cholesterol Mixed Monolavers at the Air/Water Interface. :: Langmuir. 1999 ; 巻 15, 番号 2. pp. 600-606.
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