Lipid membrane effect on the elasticity of gelatin microgel prepared inside lipid microdroplets

Atsushi Sakai, Naomi Hiro-oka, Saori Sasaki, Satoru Kidoaki, Miho Yanagisawa

研究成果: ジャーナルへの寄稿記事

抄録

In a previous study (Sakai A., et al., ACS Cent. Sci., 4, 477 (2018)), a spherical microgel of gelatin prepared inside a lipid droplet was reported to have a higher surface elasticity than the bulk gel. In this study, we investigate the role of contact or lack of contact between gelatin and the lipid membrane as well as the micrometric confinement to isolate the dominant cause of this higher elasticity of microgels. For our experiment, we prepared a concave microgel of gelatin with two surfaces, with one surface in contact with the lipid membrane and the other without being in contact with the membrane. Next, we measured the elasticities of both the surfaces by using micropipette aspiration. Although the elasticity of the surface not in contact with the lipid membrane was slightly lower than that of the surface in contact with the membrane, the elasticity value was much higher than that for the bulk gel. Further, it was found that the droplet confinement without lipids did not decrease the elasticity of gelatin microgels. These results demonstrate that the dominant factor responsible for the higher elasticity of gelatin microgels is micrometric confinement and not their contact with the lipid membrane.

元の言語英語
ページ(範囲)55-59
ページ数5
ジャーナルNihon Reoroji Gakkaishi
47
発行部数2
DOI
出版物ステータス出版済み - 1 1 2019

Fingerprint

gelatins
Gelatin
Membrane Lipids
Lipids
lipids
Elasticity
elastic properties
membranes
Gels
gels
Membranes
vacuum
causes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

これを引用

Lipid membrane effect on the elasticity of gelatin microgel prepared inside lipid microdroplets. / Sakai, Atsushi; Hiro-oka, Naomi; Sasaki, Saori; Kidoaki, Satoru; Yanagisawa, Miho.

:: Nihon Reoroji Gakkaishi, 巻 47, 番号 2, 01.01.2019, p. 55-59.

研究成果: ジャーナルへの寄稿記事

@article{f565172d2cd045f2b66e6d771e59cfc8,
title = "Lipid membrane effect on the elasticity of gelatin microgel prepared inside lipid microdroplets",
abstract = "In a previous study (Sakai A., et al., ACS Cent. Sci., 4, 477 (2018)), a spherical microgel of gelatin prepared inside a lipid droplet was reported to have a higher surface elasticity than the bulk gel. In this study, we investigate the role of contact or lack of contact between gelatin and the lipid membrane as well as the micrometric confinement to isolate the dominant cause of this higher elasticity of microgels. For our experiment, we prepared a concave microgel of gelatin with two surfaces, with one surface in contact with the lipid membrane and the other without being in contact with the membrane. Next, we measured the elasticities of both the surfaces by using micropipette aspiration. Although the elasticity of the surface not in contact with the lipid membrane was slightly lower than that of the surface in contact with the membrane, the elasticity value was much higher than that for the bulk gel. Further, it was found that the droplet confinement without lipids did not decrease the elasticity of gelatin microgels. These results demonstrate that the dominant factor responsible for the higher elasticity of gelatin microgels is micrometric confinement and not their contact with the lipid membrane.",
author = "Atsushi Sakai and Naomi Hiro-oka and Saori Sasaki and Satoru Kidoaki and Miho Yanagisawa",
year = "2019",
month = "1",
day = "1",
doi = "10.1678/rheology.47.55",
language = "English",
volume = "47",
pages = "55--59",
journal = "Nihon Reoroji Gakkaishi",
issn = "0387-1533",
publisher = "Society of Rheology",
number = "2",

}

TY - JOUR

T1 - Lipid membrane effect on the elasticity of gelatin microgel prepared inside lipid microdroplets

AU - Sakai, Atsushi

AU - Hiro-oka, Naomi

AU - Sasaki, Saori

AU - Kidoaki, Satoru

AU - Yanagisawa, Miho

PY - 2019/1/1

Y1 - 2019/1/1

N2 - In a previous study (Sakai A., et al., ACS Cent. Sci., 4, 477 (2018)), a spherical microgel of gelatin prepared inside a lipid droplet was reported to have a higher surface elasticity than the bulk gel. In this study, we investigate the role of contact or lack of contact between gelatin and the lipid membrane as well as the micrometric confinement to isolate the dominant cause of this higher elasticity of microgels. For our experiment, we prepared a concave microgel of gelatin with two surfaces, with one surface in contact with the lipid membrane and the other without being in contact with the membrane. Next, we measured the elasticities of both the surfaces by using micropipette aspiration. Although the elasticity of the surface not in contact with the lipid membrane was slightly lower than that of the surface in contact with the membrane, the elasticity value was much higher than that for the bulk gel. Further, it was found that the droplet confinement without lipids did not decrease the elasticity of gelatin microgels. These results demonstrate that the dominant factor responsible for the higher elasticity of gelatin microgels is micrometric confinement and not their contact with the lipid membrane.

AB - In a previous study (Sakai A., et al., ACS Cent. Sci., 4, 477 (2018)), a spherical microgel of gelatin prepared inside a lipid droplet was reported to have a higher surface elasticity than the bulk gel. In this study, we investigate the role of contact or lack of contact between gelatin and the lipid membrane as well as the micrometric confinement to isolate the dominant cause of this higher elasticity of microgels. For our experiment, we prepared a concave microgel of gelatin with two surfaces, with one surface in contact with the lipid membrane and the other without being in contact with the membrane. Next, we measured the elasticities of both the surfaces by using micropipette aspiration. Although the elasticity of the surface not in contact with the lipid membrane was slightly lower than that of the surface in contact with the membrane, the elasticity value was much higher than that for the bulk gel. Further, it was found that the droplet confinement without lipids did not decrease the elasticity of gelatin microgels. These results demonstrate that the dominant factor responsible for the higher elasticity of gelatin microgels is micrometric confinement and not their contact with the lipid membrane.

UR - http://www.scopus.com/inward/record.url?scp=85065960903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065960903&partnerID=8YFLogxK

U2 - 10.1678/rheology.47.55

DO - 10.1678/rheology.47.55

M3 - Article

AN - SCOPUS:85065960903

VL - 47

SP - 55

EP - 59

JO - Nihon Reoroji Gakkaishi

JF - Nihon Reoroji Gakkaishi

SN - 0387-1533

IS - 2

ER -