Lipid profiling by supercritical fluid chromatography/mass spectrometry

Takayuki Yamada, Takeshi Bamba

研究成果: Chapter in Book/Report/Conference proceedingChapter

6 被引用数 (Scopus)

抄録

Supercritical fluid chromatography (SFC) is a promising separation technique for comprehensive lipid profiling, or lipidomics. SFC facilitates superior high-throughput separation as compared to liquid chromatography (LC), and can be applied for the simultaneous profiling of diverse lipids with a wide range of polarities due to the physicochemical properties of its mobile phase. To date, analytical methods for fatty acyl, glycerolipid, glycerophospholipid, sphingolipid, sterol lipid, and prenol lipid profiling using SFC coupled to mass spectrometry (MS) have been developed. Hundreds of different molecular species have been identified from biological samples by combining SFC/MS and bioinformatics tools. In addition, SFC can be connected directly with supercritical fluid extraction (SFE), which is suitable for the efficient recovery of hydrophobic compounds that are susceptible to photolytic degradation and oxidation. Online SFE-SFC/MS can be utilized for the high-throughput analysis of the redox status of coenzyme Q10 (CoQ10), and phospholipids in dried plasma spots (DPS). These techniques have potential applications in the high-throughput screening of a large number of samples, in biomarker discovery, and in the elucidation of lipid metabolism mechanisms based on the functions of individual lipid molecular species.

本文言語英語
ホスト出版物のタイトルNeuromethods
出版社Humana Press Inc.
ページ109-131
ページ数23
DOI
出版ステータス出版済み - 3 1 2017

出版物シリーズ

名前Neuromethods
125
ISSN(印刷版)0893-2336
ISSN(電子版)1940-6045

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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