Lipidated Brartemicin Analogues Are Potent Th1-Stimulating Vaccine Adjuvants

Amy J. Foster, Masahiro Nagata, Xiuyuan Lu, Amy T. Lynch, Zakaria Omahdi, Eri Ishikawa, Sho Yamasaki, Mattie S.M. Timmer, Bridget L. Stocker

    研究成果: Contribution to journalArticle査読

    16 被引用数 (Scopus)

    抄録

    Effective Th1-stimulating vaccine adjuvants typically activate antigen presenting cells (APCs) through pattern recognition receptors (PRRs). Macrophage inducible C-type lectin (Mincle) is a PRR expressed on APCs and has been identified as a target for Th1-stimulating adjuvants. Herein, we report on the synthesis and adjuvanticity of rationally designed brartemicin analogues containing long-chain lipids and demonstrate that they are potent Mincle agonists that activate APCs to produce inflammatory cytokines in a Mincle-dependent fashion. Mincle binding, however, does not directly correlate to a functional immune response. Mutation studies indicated that the aromatic residue of lead compound 9a has an important interaction with Mincle Arg183. In vivo assessment of 9a highlighted the capability of this analogue to augment the Th1 response to a model vaccine antigen. Taken together, our results show that lipophilic brartemicin analogues are potent Mincle agonists and that 9a has superior in vivo adjuvant activity compared to TDB.

    本文言語英語
    ページ(範囲)1045-1060
    ページ数16
    ジャーナルJournal of Medicinal Chemistry
    61
    3
    DOI
    出版ステータス出版済み - 2 8 2018

    All Science Journal Classification (ASJC) codes

    • Molecular Medicine
    • Drug Discovery

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