Liquid chromatography-fast atom bombardment mass spectrometry for detection and determination of pentazocine in human tissues

Tohru Imamura, Keiko Kudo, Akira Namera, Hironori Tokunaga, Mikio Yashiki, Narumi Jitsufuchi, Tohru Kojima

研究成果: Contribution to journalArticle

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A reliable and sensitive method was developed for the detection and determination of pentazocine in human solid tissues using liquid chromatography-dynamic fast atom bombardment (FAB) mass spectrometry, combined with a three-step liquid-liquid extraction procedure. Levallorphan tartrate served as an internal standard. The extract was evaporated to dryness and dissolved in the mobile phase, acetonitrile-10 mM ammonium acetate solution (20:80, pH 4.0) containing 0.5% glycerol as FAB matrix. The eluent was pumped at a flow rate of 25 μl/min and split before introduction to FAB mass spectrometer. Quantitative analysis was carried out by means of monitoring quasi-molecular ions with m/z 286 for pentazocine and m/z 284 for levallorphan. The lower limit of detection of pentazocine in each tissue tested was 1 ng/g with scan mode and 0.1 ng/g with SIM mode. Using this method, the concentrations of pentazocine were determined in the tissues of an autopsied individual to perform toxicological evaluation. Copyright (C) 1999 Elsevier Science B.V.

元の言語英語
ページ(範囲)149-154
ページ数6
ジャーナルJournal of Chromatography B: Biomedical Sciences and Applications
731
発行部数1
DOI
出版物ステータス出版済み - 8 6 1999

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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