Liquid-phase detection of biological targets with magnetic markers and high Tc SQUID

S. Uchida, Y. Higuchi, Y. Ueoka, T. Yoshida, K. Enpuku, S. Adachi, K. Tanabe, A. Tsukamoto

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

1 被引用数 (Scopus)

抄録

We developed a highly sensitive HTS SQUID system for liquid-phase detection of biological targets using Brownian relaxation of magnetic markers. The SQUID was made of a ramp-edge Josephson junction using RE123-based multilayer process, and it showed a flux noise of 7.5 micro flux-quantam/Hz1/2 in the white noise region. The 1/f noise was 14 micro flux-quantam/Hz1/2 at f = 1 Hz when the SQUID was operated with AC bias mode. Using the SQUID system, we detected a biological target called biotin. In the experiment, biotins were fixed to a large polymer bead with a diameter of 3.3 micron meter. Streptavidin-coated magnetic markers were used for the detection. The bound and unbound (free) markers were magnetically distinguished using the difference in their Brownian relaxation time, i.e., without using the washing process to separate them. The minimum detectable number of biotins was as low as 10 4, corresponding to a sensitivity of 4.8×10-19 mol/ml. This result indicates a high sensitivity of the developed method.

本文言語英語
ホスト出版物のタイトル2013 IEEE 14th InternationalSuperconductive Electronics Conference, ISEC 2013
DOI
出版ステータス出版済み - 10 15 2013
イベント2013 IEEE 14th InternationalSuperconductive Electronics Conference, ISEC 2013 - Cambridge, MA, 米国
継続期間: 7 7 20137 11 2013

出版物シリーズ

名前2013 IEEE 14th InternationalSuperconductive Electronics Conference, ISEC 2013

その他

その他2013 IEEE 14th InternationalSuperconductive Electronics Conference, ISEC 2013
Country米国
CityCambridge, MA
Period7/7/137/11/13

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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