Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages

Masaharu Murata, Sayoko Narahara, Kaori Umezaki, Riki Toita, Shigekazu Tabata, Jing Shu Piao, Kana Abe, Jeong Hun Kang, Kenoki Ohuchida, Lin Cui, Makoto Hashizume

研究成果: ジャーナルへの寄稿学術誌査読

23 被引用数 (Scopus)

抄録

Protein nanocages are self-organized complexes of oligomers whose three-dimensional architecture can been determined in detail. These structures possess nanoscale inner cavities into which a variety of molecules, including therapeutic or diagnostic agents, can be encapsulated. These properties yield these particles suitable for a new class of drug delivery carrier, or as a bioimaging reagent that might respond to biochemical signals in many different cellular processes. We report here the design, synthesis, and biological characterization of a hepatocyte-specific nanocage carrying small heat-shock protein. These nanoscale protein cages, with a targeting peptide composed of a preS1 derivative from the hepatitis B virus on their surfaces, were prepared by genetic engineering techniques. PreS1-carrying nanocages showed lower cytotoxicity and significantly higher specificity for human hepatocyte cell lines than other cell lines in vitro. These results suggested that small heat-shock protein-based nanocages present great potential for the development of effective targeted delivery of various agents to specific cells.

本文言語英語
ページ(範囲)4353-4362
ページ数10
ジャーナルInternational journal of nanomedicine
7
DOI
出版ステータス出版済み - 2012

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • バイオエンジニアリング
  • 生体材料
  • 薬科学
  • 創薬
  • 有機化学

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