Localization of an Aldo-Keto Reductase (AKR2E4) in the Silkworm Bombyx mori (Lepidoptera: Bombycidae)

K. Yamamoto, Y. Ozakiya, T. Uno

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

The aldo-keto reductase AKR2E4 reduces 3-dehydroecdysone to ecdysone in the silkworm Bombyx mori L. In this study, a quantitative polymerase chain reaction analysis revealed that the level of AKR2E4 mRNA was higher in the testes than in other tissues, and a western immunoblot analysis revealed that the AKR2E4 content in the testes was stage-specific from the fifth larval instar to the pupal stage. Immunohistochemical analysis showed that the AKR2E4 protein was present in cyst cells associated with sperm cells and spermatocytes. These results indicate that AKR2E4 plays an important role in 3-dehydroecdysone conversion to ecdysone and spermatogenesis in silkworm testes.

本文言語英語
論文番号94
ジャーナルJournal of Insect Science
17
5
DOI
出版ステータス出版済み - 9 1 2017

All Science Journal Classification (ASJC) codes

  • 昆虫科学

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