Localization of metallothionein in nuclei of growing primary cultured adult rat hepatocytes

Kazutake Tsujikawa, Takumi Imai, Makoto Kakutani, Yuzo Kayamori, Tsutomu Mimura, Noriko Otaki, Masami Kimura, Ryuichi Fukuyama, Nobuyoshi Shimizu

研究成果: Contribution to journalArticle査読

97 被引用数 (Scopus)

抄録

In primary cultured adult rat hepatocytes stimulated by epidermal growth factor and insulin, dramatic changes in the subcellular distribution of metallothionein were clarified by indirect immunofluorescence using antisera specific for this protein. Metallothionein was detected only in the cytoplasm of cultured hepatocytes in the G0 and G1 phases, but was concentrated in the cell nuclei in the early S phase. The strongest staining pattern in the nuclei was observed 12 h after stimulation. Subsequently, the intensity of metallothionein staining in the nuclei decreased. These results suggests that primary cultured hepatocytes are suitable for examining the relation between subcellular localization of metallothionein and cell growth.

本文言語英語
ページ(範囲)239-242
ページ数4
ジャーナルFEBS Letters
283
2
DOI
出版ステータス出版済み - 6 3 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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