LONG GRAIN 1: a novel gene that regulates grain length in rice

Ryouhei Morita, Hiroyuki Ichida, Kotaro Ishii, Yoriko Hayashi, Hiroshi Abe, Yuki Shirakawa, Katsunori Ichinose, Kazuhide Tsuneizumi, Tomohiko Kazama, Kinya Toriyama, Tadashi Sato, Tomoko Abe

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

Grain size is one of the most important traits that influence grain yield in rice. Here we report the isolation and characterization of the long grain1 (lin1) mutant, which exhibited long grains and increased grain weight. A field trial conducted over 2 years indicated that the grain weight increased 3.0–6.6% in the lin1 mutant compared with that of the wild-type Nipponbare. Whole-genome sequencing and genetic linkage analysis indicated that a 1-bp deletion within the coding sequence of Os06g0675200, which showed no homology to previously characterized genes, is responsible for the lin1 phenotype. Quantitative real-time PCR indicated that LIN1 transcripts were more abundant in young panicles than at advanced inflorescence developmental stages, which indicated that LIN1 controls grain length mainly at early stages of grain development. Sequence polymorphism analysis of LIN1 showed that all 15 temperate japonica cultivars tested as well as six out of the nine indica cultivars tested possessed the Nipponbare-type LIN1 allele, whereas the remaining three indica cultivars and one aus cultivar tested harbored an identical missense mutation in LIN1. These results revealed that the mutant allele of LIN1 has not been widely utilized in breeding temperate japonica cultivars currently in cultivation. Our findings indicate that the lin1 mutation may be useful to further improve grain length and presumably grain yield in temperate japonica and indica rice cultivars that harbor the Nipponbare-type LIN1 allele.

本文言語英語
論文番号135
ジャーナルMolecular Breeding
39
9
DOI
出版ステータス出版済み - 9 1 2019
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分子生物学
  • 農業および作物学
  • 遺伝学
  • 植物科学

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