Long-term preservation of eri and ailanthus silkworms using frozen gonads

Hisayoshi Fukumori, Jung Lee, Tsuguru Fujii, Zenta Kajiura, Yutaka Banno

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Cryopreservation of eri and ailanthus silkworms using frozen gonads was investigated. First, we evaluated the freeze tolerance of ovary and testis in the eri silkworm, which showed high tolerance. Mating between frozen ovary-transplanted females and frozen testis-transplanted males produced 163.0 eggs, yielding 105.7 larvae per moth. In a second experiment, we tested the use of the eri silkworm as a host insect for gonad transplantation from ailanthus silkworm donors. A high success ratio for laid and hatched eggs was demonstrated for ovary transplantation (97.8 and 51.3 eggs per moth, respectively). For testis transplantation, however, the average number of hatched larvae was low (12.0). Mating between host eri females and males in which both frozen ovary and testis of the ailanthus silkworm had been transplanted produced 6.4 fertilized eggs per host moth. Our success in using cross subspecies cryopreservation between these wild silkworms could lead to the alternative use of hosts between species in other insects.

元の言語英語
ページ(範囲)71-74
ページ数4
ジャーナルCryobiology
77
DOI
出版物ステータス出版済み - 8 1 2017

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Ailanthus
Bombyx
Gonads
silkworms
gonads
testes
Moths
Testis
Ovary
moths
Eggs
Transplantation
cryopreservation
Cryopreservation
Larva
Insects
insects
larvae
cold tolerance
Zygote

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Long-term preservation of eri and ailanthus silkworms using frozen gonads. / Fukumori, Hisayoshi; Lee, Jung; Fujii, Tsuguru; Kajiura, Zenta; Banno, Yutaka.

:: Cryobiology, 巻 77, 01.08.2017, p. 71-74.

研究成果: ジャーナルへの寄稿記事

Fukumori, Hisayoshi ; Lee, Jung ; Fujii, Tsuguru ; Kajiura, Zenta ; Banno, Yutaka. / Long-term preservation of eri and ailanthus silkworms using frozen gonads. :: Cryobiology. 2017 ; 巻 77. pp. 71-74.
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