Loqs depends on R2D2 to localize in D2 body-like granules and functions in RNAi pathways in silkworm cells

Li Zhu, Tsuneyuki Tatsuke, Jian Xu, Zhiqing Li, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

The phenomenon of RNA interference (RNAi) has been found in various organisms. However, the proteins implicated in RNAi pathway in different species show distinct roles. Knowledge on the underlying mechanism of lepidopteron RNAi is quite lacking such as the roles of Loquacious (Loqs) and R2D2, the dsRNA-binding proteins in silkworm RNAi pathway. Here, we report that Loqs and R2D2 protein depletion affected efficiency of dsRNA-mediated RNAi pathway. Besides, Loqs was found to co-localize with Dicer2 to some specific cytoplasmic foci, which were looked like D2-bodies marked by R2D2 and Dicer2 in Fly cells, thereby calling the foci as D2 body-like granules. Using RNAi methods, Loqs was found to be the key protein in these granules, although R2D2 determined the localization of Loqs in D2 body-like granules. Interestingly, in the R2D2-depeted silkworm cells, the formation of processing bodies, another cytoplasmic foci, was affected. These data indicated R2D2 regulated these two kinds of cytoplasmic foci. Domain deletion analysis demonstrated that dsRBD 1 and 2 were required for Loqs in D2 body-like granules and dsRBD 2 and 3 were required for Loqs to interact with R2D2 and Ago1, respectively. Altogether, our observations provide important information for further study on D2 body-like granules, the newly found cytoplasmic foci in silkworm cells.

本文言語英語
ページ(範囲)78-90
ページ数13
ジャーナルInsect Biochemistry and Molecular Biology
64
DOI
出版ステータス出版済み - 9 1 2015

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 昆虫科学

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