Loss of protein kinase C delta alters mammary gland development and apoptosis

B. L. Allen-Petersen, M. R. Miller, M. C. Neville, S. M. Anderson, Keiichi Nakayama, M. E. Reyland

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

As apoptotic pathways are commonly deregulated in breast cancer, exploring how mammary gland cell death is regulated is critical for understanding human disease. We show that primary mammary epithelial cells from protein kinase C delta (PKC) / mice have a suppressed response to apoptotic agents in vitro. In the mammary gland in vivo, apoptosis is critical for ductal morphogenesis during puberty and involution following lactation. We have explored mammary gland development in the PKC / mouse during these two critical windows. Branching morphogenesis was altered in 4- to 6-week-old PKC / mice as indicated by reduced ductal branching; however, apoptosis and proliferation in the terminal end buds was unaltered. Conversely, activation of caspase-3 during involution was delayed in PKC / mice, but involution proceeded normally. The thymus also undergoes apoptosis in response to physiological signals. A dramatic suppression of caspase-3 activation was observed in the thymus of PKC / mice treated with irradiation, but not mice treated with dexamethasone, suggesting that there are both target- and tissue-dependent differences in the execution of apoptotic pathways in vivo. These findings highlight a role for PKC in both apoptotic and nonapoptotic processes in the mammary gland and underscore the redundancy of apoptotic pathways in vivo.

元の言語英語
記事番号e17
ジャーナルCell Death and Disease
1
発行部数1
DOI
出版物ステータス出版済み - 1 1 2010

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Protein Kinase C-delta
Human Mammary Glands
Apoptosis
Morphogenesis
Caspase 3
Thymus Gland
Breast Neoplasms
Puberty
Lactation
Dexamethasone
Breast
Cell Death
Epithelial Cells
Mouse Prkcd protein

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

これを引用

Allen-Petersen, B. L., Miller, M. R., Neville, M. C., Anderson, S. M., Nakayama, K., & Reyland, M. E. (2010). Loss of protein kinase C delta alters mammary gland development and apoptosis. Cell Death and Disease, 1(1), [e17]. https://doi.org/10.1038/cddis.2009.20

Loss of protein kinase C delta alters mammary gland development and apoptosis. / Allen-Petersen, B. L.; Miller, M. R.; Neville, M. C.; Anderson, S. M.; Nakayama, Keiichi; Reyland, M. E.

:: Cell Death and Disease, 巻 1, 番号 1, e17, 01.01.2010.

研究成果: ジャーナルへの寄稿記事

Allen-Petersen, BL, Miller, MR, Neville, MC, Anderson, SM, Nakayama, K & Reyland, ME 2010, 'Loss of protein kinase C delta alters mammary gland development and apoptosis', Cell Death and Disease, 巻. 1, 番号 1, e17. https://doi.org/10.1038/cddis.2009.20
Allen-Petersen, B. L. ; Miller, M. R. ; Neville, M. C. ; Anderson, S. M. ; Nakayama, Keiichi ; Reyland, M. E. / Loss of protein kinase C delta alters mammary gland development and apoptosis. :: Cell Death and Disease. 2010 ; 巻 1, 番号 1.
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