Low cytotoxic gene delivery system by a polysaccharide-poly(dA) tailed DNA complex

Kazuo Sakurai, Takahisa Anada, Ryouji Karinaga, Emi Hamada, Kazuya Koumoto, Masami Mizu, Seiji Shinkai

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)80 tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture that is quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation. We have evaluated the performance for SPG/poly(dA) tailed DNA and other related complexes.

本文言語英語
ホスト出版物のタイトル54th SPSJ Annual Meeting 2005 - Polymer Preprints, Japan
ページ2141
ページ数1
54
1
出版ステータス出版済み - 2005
イベント54th SPSJ Annual Meeting 2005 - Yokohama, 日本
継続期間: 5 25 20055 27 2005

その他

その他54th SPSJ Annual Meeting 2005
Country日本
CityYokohama
Period5/25/055/27/05

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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