Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification

Wan Kin Au Yeung, Hiroyuki Sasaki

研究成果: 書籍/レポート タイプへの寄稿

抄録

Whole genome bisulfite sequencing (WGBS) is a high-throughput DNA sequencing-based technique that is used to determine genome-wide DNA methylation patterns at base resolution. Library construction by post-bisulfite adaptor tagging (PBAT) extends the application of WGBS to several hundred cells and minimizes the required number of library amplification cycles. We herein describe a PBAT protocol to prepare WGBS libraries from 200 cells and introduce the outline of a downstream bioinformatic analysis. The prepared library can typically generate 800 million sequencing reads, which is sufficient to cover the human and mouse genomes approximately 15 times, using the Illumina NovaSeq 6000 sequencing system.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ233-250
ページ数18
DOI
出版ステータス出版済み - 2022

出版物シリーズ

名前Methods in Molecular Biology
2509
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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