レタス(Lactuca sativa L.)プロトプラストへのイオンビーム照射によって出現した低ポリフェノールオキシダーゼ活性を示す突然変異体

澤田 倫平, 平野 智也, 飯牟禮 和彦, 阿部 知子, 尾崎 行生

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<p>The induction of polyphenol oxidase (PPO) gene mutation through ion beam irradiation of protoplasts was attempted for the production of lettuce plants with a low browning property as a cut vegetable. It was clarified that the optimum protoplast density was 0.63–0.75 × 10<sup>4</sup> mL<sup>–1</sup> for shoot regeneration from protoplasts of lettuce 'Round'. Effects of <sup>12</sup>C<sup>6+</sup> ion beam irradiation on the growth of protoplasts were investigated, and the percentage of colony formation decreased as the irradiation dose increased. The dose leading to 50% cell growth as compared with the untreated control was approximately 3.3 Gy. Two out of 869 calli irradiated with <sup>12</sup>C<sup>6+</sup> ion beams were found to be <i>PPO</i> gene mutations by the TILLING method. PPO activities of the regenerated plants from the two mutant calli named 2Gy-3-36 and 5Gy-2-27 were preliminarily assayed, and lettuce plants from 2Gy-3-36 exhibited half of the PPO activity as compared with the non-irradiated controls. A part of the M<sub>2</sub> population from 2Gy-2-36 exhibited the property of no band of heteroduplex cleavage by <i>Cel</i> I, with markedly low PPO activity as the control.</p>
寄稿の翻訳されたタイトルLow Polyphenol Oxidase Mutant Induced by <sup>12</sup>C<sup>6+</sup> Ion Beam Irradiation to Protoplasts of Lettuce (<i>Lactuca sativa</i> L.)
元の言語Japanese
ページ(範囲)347-353
ページ数7
ジャーナル園芸学研究
15
発行部数4
DOI
出版物ステータス出版済み - 2016

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