Lysosomal acid phosphatase is transported via endosomes to lysosomes

Yoshitaka Tanaka, Shinji Yano, Kazutaka Okada, Toyoko Ishikawa, Masaru Himeno, Keitaro Kato

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

Involvement of endosomes in transport of newly synthesized acid phosphatase to lysosomes was investigated using the Golgi fraction (GF1+2), enriched in endosomes. The Golgi fraction (GF1+2) was prepared from the livers of rats given [35S]methionine and asialofetuin conjugated-horseradish peroxidase (HRP). Newly synthesized acid phosphatase in the endosomes containing internalized asialofetuin-HRP was measured as a loss of the detectable labeled enzyme after 3,3′-diaminobenzidine (DAB) and H2O2 reaction, due to formation of insoluble polymers which reduce protein antigenicity. With this procedure, acid phosphatase was all but undetectable in the Golgi fraction. Thus, newly synthesized acid phosphatase is apparently transported to lysosomes by endosomes.

本文言語英語
ページ(範囲)1176-1182
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
166
3
DOI
出版ステータス出版済み - 2 14 1990

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Lysosomal acid phosphatase is transported via endosomes to lysosomes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル