Macrophage colony-stimulating factor and interleukin-1 alpha maintain the survival of osteoclast-like cells

Eijiro Jimi, Toshihide Shuto, Toshitaka Koga

研究成果: ジャーナルへの寄稿学術誌査読

162 被引用数 (Scopus)

抄録

When mouse bone marrow cells were co-cultured with a stromal cell line. ST2, numerous osteoclast-like multinucleated cells (MNCs) were formed. To enrich the MNCs which were tartrate-resistant acid phosphatase (TRAP)-positive, we treated the cultures with dispase. Enriched TRAP-positive MNCs adhering to the bottom of the dish were cultured for a further 24h with medium containing 15% fetal calf serum. More than 80% of TRAP-positive MNCs were detached from the dish during the culture. However, when ST2 cells were included during the culture period, survival of TRAP-positive MNCs were significantly increased. Moreover, among various factors examined for the survival of TRAP-positive MNCs, only M-CSF and IL-1 alpha were effective in prolonging viability of TRAP-positive MNCs.

本文言語英語
ページ(範囲)808-811
ページ数4
ジャーナルEndocrinology
136
2
DOI
出版ステータス出版済み - 2月 1995

!!!All Science Journal Classification (ASJC) codes

  • 内分泌学

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