Magnetic manipulation of a retroviral vector using magnetite cationic liposomes

Akira Ito, Tetsuya Takahashi, Yujiro Kameyama, Kawabe Yoshinori, Masamichi Kamihira

研究成果: 書籍/レポート タイプへの寄稿会議への寄与

抄録

In the present study, we used magnetite nanoparticles and magnetic force to concentrate the retroviral vectors in order to enhance the transduction efficiency and to enable their magnetic manipulation. Magnetite nanoparticles modified with Cationic liposomes were added to a solution containing a retroviral vector. The magnetic particles which captured the viral vectors were collected by a magnetic force, and seeded into target cells. The viral titer increased up to 55-fold and 3 x 108 IU/mL. Additionally, the magnetically labeled retroviral vectors can be directed to the desired regions for infection by applying magnetic fields, and micro-patterns of gene-transduced cell regions could be created on a cellular monolayer using micro-patterned magnetic concentrators. These results suggest that this new technique provides a promising approach to capture and concentrate viral vectors, thus achieving high transduction efficiency and the ability to deliver genes to a specifically injured site by applying a magnetic field.

本文言語英語
ホスト出版物のタイトル2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
ページ367-371
ページ数5
DOI
出版ステータス出版済み - 12月 1 2008
イベント2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, 日本
継続期間: 11月 6 200811月 9 2008

出版物シリーズ

名前2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

その他

その他2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
国/地域日本
CityNagoya
Period11/6/0811/9/08

!!!All Science Journal Classification (ASJC) codes

  • コンピュータ ビジョンおよびパターン認識
  • 人間とコンピュータの相互作用
  • 電子工学および電気工学

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