Maitotoxin-photoactive probe binds to membrane proteins in blood cells

Keiichi Konoki, Masaki Hashimoto, Kaori Honda, Kazuo Tachibana, Rie Tamate, Futoshi Hasegawa, Tohru Oishi, Michio Murata

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

The photoactive and biotinylating ligand was prepared from MTX and maleimide-conjugated Hatanaka reagent with use of Diels-Alder reaction. Blood cells were subjected to affinity labelling experiments using the ligand thus obtained. The labelled band on SDS-PAGE was replaced not by MTX but by brevetoxin B (PbTx2), which suggested the presence of binding proteins in blood 2+ cells. Screening of polyether compounds for MTX inhibitory activity using Ca flux assays in C6 cells disclosed that a synthetic fragment of the hydrophilic portion of MTX inhibited the MTX activity.

元の言語英語
ページ(範囲)1007-1017
ページ数11
ジャーナルHeterocycles
79
発行部数C
DOI
出版物ステータス出版済み - 7 29 2009
外部発表Yes

Fingerprint

Blood Cells
Membrane Proteins
Blood
Cells
Ligands
Polyethers
Cycloaddition Reaction
Labeling
Polyacrylamide Gel Electrophoresis
Assays
Carrier Proteins
Screening
Fluxes
Experiments
maitotoxin
maleimide
brevetoxin B

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmacology
  • Organic Chemistry

これを引用

Konoki, K., Hashimoto, M., Honda, K., Tachibana, K., Tamate, R., Hasegawa, F., ... Murata, M. (2009). Maitotoxin-photoactive probe binds to membrane proteins in blood cells. Heterocycles, 79(C), 1007-1017. https://doi.org/10.3987/COM-08-S(D)78

Maitotoxin-photoactive probe binds to membrane proteins in blood cells. / Konoki, Keiichi; Hashimoto, Masaki; Honda, Kaori; Tachibana, Kazuo; Tamate, Rie; Hasegawa, Futoshi; Oishi, Tohru; Murata, Michio.

:: Heterocycles, 巻 79, 番号 C, 29.07.2009, p. 1007-1017.

研究成果: ジャーナルへの寄稿記事

Konoki, K, Hashimoto, M, Honda, K, Tachibana, K, Tamate, R, Hasegawa, F, Oishi, T & Murata, M 2009, 'Maitotoxin-photoactive probe binds to membrane proteins in blood cells', Heterocycles, 巻. 79, 番号 C, pp. 1007-1017. https://doi.org/10.3987/COM-08-S(D)78
Konoki K, Hashimoto M, Honda K, Tachibana K, Tamate R, Hasegawa F その他. Maitotoxin-photoactive probe binds to membrane proteins in blood cells. Heterocycles. 2009 7 29;79(C):1007-1017. https://doi.org/10.3987/COM-08-S(D)78
Konoki, Keiichi ; Hashimoto, Masaki ; Honda, Kaori ; Tachibana, Kazuo ; Tamate, Rie ; Hasegawa, Futoshi ; Oishi, Tohru ; Murata, Michio. / Maitotoxin-photoactive probe binds to membrane proteins in blood cells. :: Heterocycles. 2009 ; 巻 79, 番号 C. pp. 1007-1017.
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