Maitotoxin-photoactive probe binds to membrane proteins in blood cells

Keiichi Konoki, Masaki Hashimoto, Kaori Honda, Kazuo Tachibana, Rie Tamate, Futoshi Hasegawa, Tohru Oishi, Michio Murata

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

The photoactive and biotinylating ligand was prepared from MTX and maleimide-conjugated Hatanaka reagent with use of Diels-Alder reaction. Blood cells were subjected to affinity labelling experiments using the ligand thus obtained. The labelled band on SDS-PAGE was replaced not by MTX but by brevetoxin B (PbTx2), which suggested the presence of binding proteins in blood 2+ cells. Screening of polyether compounds for MTX inhibitory activity using Ca flux assays in C6 cells disclosed that a synthetic fragment of the hydrophilic portion of MTX inhibited the MTX activity.

本文言語英語
ページ(範囲)1007-1017
ページ数11
ジャーナルHeterocycles
79
C
DOI
出版ステータス出版済み - 7 29 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 薬理学
  • 有機化学

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