Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice

Amédée Des Georges, Miho Katsuki, Douglas Robert Drummond, Michael Osei, Robert A. Cross, Linda A. Amos

研究成果: ジャーナルへの寄稿記事

77 引用 (Scopus)

抄録

In vitro studies of pure tubulin have suggested that tubulin heterodimers in cells assemble into B-lattice microtubules, where the 8-nm dimers in adjacent protofilaments are staggered by 0.9 nm. This arrangement requires the tube to close by forming a seam with an A-lattice, in which the protofilaments are staggered by 4.9 nm. Here we show that Mal3, an EB1 family tip-tracking protein, drives tubulin to assemble in vitro into exclusively 13-protofilament microtubules with a high proportion of A-lattice protofilament contacts. We present a three-dimensional cryo-EM reconstruction of a purely A-lattice microtubule decorated with Mal3, in which Mal3 occupies the groove between protofilaments and associates closely with one tubulin monomer. We propose that Mal3 promotes assembly by binding to freshly formed tubulin polymer and particularly favors any with A-lattice arrangement. These results reopen the question of microtubule structure in cells.

元の言語英語
ページ(範囲)1102-1108
ページ数7
ジャーナルNature Structural and Molecular Biology
15
発行部数10
DOI
出版物ステータス出版済み - 10 1 2008
外部発表Yes

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Schizosaccharomyces
Tubulin
Microtubules
Polymers
Proteins
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

これを引用

Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice. / Des Georges, Amédée; Katsuki, Miho; Drummond, Douglas Robert; Osei, Michael; Cross, Robert A.; Amos, Linda A.

:: Nature Structural and Molecular Biology, 巻 15, 番号 10, 01.10.2008, p. 1102-1108.

研究成果: ジャーナルへの寄稿記事

Des Georges, Amédée ; Katsuki, Miho ; Drummond, Douglas Robert ; Osei, Michael ; Cross, Robert A. ; Amos, Linda A. / Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice. :: Nature Structural and Molecular Biology. 2008 ; 巻 15, 番号 10. pp. 1102-1108.
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AU - Cross, Robert A.

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