MALDI efficiency of metabolites quantitatively associated with their structural properties: A quantitative structure-property relationship (QSPR) approach

Daichi Yukihira, Daisuke Miura, Yoshinori Fujimura, Yoshikatsu Umemura, Shinichi Yamaguchi, Shinji Funatsu, Makoto Yamazaki, Tetsuya Ohta, Hiroaki Inoue, Mitsuru Shindo, Hiroyuki Wariishi

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) experiments require a suitable match of the matrix and target compounds to achieve a selective and sensitive analysis. However, it is still difficult to predict which metabolites are ionizable with a given matrix and which factors lead to an efficient ionization. In the present study, we extracted structural properties of metabolites that contribute to their ionization in MALDI-MS analyses exploiting our experimental data set. The MALDI-MS experiment was performed for 200 standard metabolites using 9-aminoacridine (9-AA) as the matrix. We then developed a prediction model for the ionization profiles (both the ionizability and ionization efficiency) of metabolites using a quantitative structure-property relationship (QSPR) approach. The classification model for the ionizability achieved a 91 % accuracy, and the regression model for the ionization efficiency reached a rank correlation coefficient of 0.77. An analysis of the descriptors contributing to such model construction suggested that the proton affinity is a major determinant of the ionization, whereas some substructures hinder efficient ionization. This study will lead to the development of more rational and predictable MALDI-MS analyses.

本文言語英語
ページ(範囲)1-5
ページ数5
ジャーナルJournal of the American Society for Mass Spectrometry
25
1
DOI
出版ステータス出版済み - 1 2014

All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 分光学

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