MALS is a binding partner of IRSp53 at cell-cell contacts

Kei Hori, Daijiro Konno, Hisato Maruoka, Kenji Sobue

研究成果: ジャーナルへの寄稿学術誌査読

26 被引用数 (Scopus)

抄録

Insulin receptor substrate p53 (IRSp53) is a key player in cytoskeletal dynamics, interacting with the actin modulators WAVE2 and Mena. Here, we identified a PDZ protein, MALS, as an IRSp53-interacting protein using a yeast two-hybrid screen. A pull-down assay showed that IRSp53 and MALS interact through the PDZ domain of MALS and the C-terminal PDZ-binding sequence of IRSp53. Their interaction in MDCK cells was also demonstrated by co-immunoprecipitation. Immunocytochemistry showed the colocalization of IRSp53 and MALS at cell-cell contacts. Cytochalasin D induced the redistribution of both proteins to the cytosol. Thus, MALS is a partner of IRSp53 anchoring the actin-based membrane cytoskeleton at cell-cell contacts.

本文言語英語
ページ(範囲)30-34
ページ数5
ジャーナルFEBS Letters
554
1-2
DOI
出版ステータス出版済み - 11月 6 2003
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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