Mammalian Elongin A is not essential for cell viability but is required for proper cell cycle progression with limited alteration of gene expression

Katsuhisa Yamazaki, Teijiro Aso, Yoshinori Ohnishi, Mizuki Ohno, Kenji Tamura, Taro Shuin, Shigetaka Kitajima, Yusaku Nakabeppu

研究成果: Contribution to journalArticle査読

14 被引用数 (Scopus)

抄録

Elongin A is a transcription elongation factor that increases the overall rate of mRNA chain elongation by RNA polymerase II. To investigate the function of Elongin A in vivo, the two alleles of the Elongin A gene have been disrupted by homologous recombination in murine embryonic stem (ES) cells. The Elongin A-deficient ES cells are viable, but show a slow growth phenotype because they undergo a delayed mitosis. The cDNA microarray and RNase protection assay using the wildo type and Elongin A-deficient ES cells indicate that the expression of only a small subset of genes is affected in the mutant cells. Taken together, our results suggest that Elongin A regulates transcription of a subset but not all of genes and reveal a linkage between Elongin A function and cell cycle progression.

本文言語英語
ページ(範囲)13585-13589
ページ数5
ジャーナルJournal of Biological Chemistry
278
15
DOI
出版ステータス出版済み - 4 11 2003

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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