Mapping of ubiquitination sites on target proteins

Shigetsugu Hatakeyama, Masaki Matsumoto, Keiichi I. Nakayama

研究成果: ジャーナルへの寄稿総説査読

9 被引用数 (Scopus)

抄録

Although the identification of ubiquitin-conjugated lysine residues on target proteins is extremely significant, to date it is generally quite difficult to identify ubiquitinated sites by usual mutation analysis. More recently, the technology of mass spectrometry is answering these difficult questions. In this chapter, we introduce the method of purification of ubiquitinated target proteins using affinity chromatography with anti-polyubiquitin antibody and the identification of ubiquitinated lysine residues on target proteins using mass spectrometry. Using these techniques, we can obtain comprehensive information about ubiquitinated proteins in various cells and tissues.

本文言語英語
論文番号19
ページ(範囲)277-286
ページ数10
ジャーナルMethods in enzymology
399
DOI
出版ステータス出版済み - 2005

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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