抄録
Key message: Greatest potential, QTLs for hypoxia and waterlogging tolerance in soybean roots were detected using a new phenotypic evaluation method. Abstract: Waterlogging is a major environmental stress limiting soybean yield in wet parts of the world. Root development is an important indicator of hypoxia tolerance in soybean. However, little is known about the genetic control of root development under hypoxia. This study was conducted to identify quantitative trait loci (QTLs) responsible for root development under hypoxia. Recombinant inbred lines (RILs) developed from a cross between a hypoxia-sensitive cultivar, Tachinagaha, and a tolerant landrace, Iyodaizu, were used. Seedlings were subjected to hypoxia, and root development was evaluated with the value change in root traits between after and before treatments. We found 230 polymorphic markers spanning 2519.2 cM distributed on all 20 chromosomes (Chrs.). Using these, we found 11 QTLs for root length (RL), root length development (RLD), root surface area (RSA), root surface area development (RSAD), root diameter (RD), and change in average root diameter (CARD) on Chrs. 11, 12, 13 and 14, and 7 QTLs for hypoxia tolerance of these root traits. These included QTLs for RLD and RSAD between markers Satt052 and Satt302 on Chr. 12, which are important markers of hypoxia tolerance in soybean; those QTLs were stable between 2 years. To validate the QTLs, we developed a near-isogenic line with the QTL region derived from Iyodaizu. The line performed well under both hypoxia and waterlogging, suggesting that the region contains one or more genes with large effects on root development. These findings may be useful for fine mapping and positional cloning of gene responsible for root development under hypoxia.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 743-755 |
| ページ数 | 13 |
| ジャーナル | Theoretical and Applied Genetics |
| 巻 | 130 |
| 発行部数 | 4 |
| DOI | |
| 出版物ステータス | 出版済み - 4 1 2017 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Agronomy and Crop Science
- Genetics
これを引用
Mapping quantitative trait loci for root development under hypoxia conditions in soybean (Glycine max L. Merr.). / Van Nguyen, Loc; Takahashi, Ryoji; Githiri, Stephen Mwangi; Rodriguez, Tito O.; Tsutsumi, Nobuko; Kajihara, Sayuri; Sayama, Takasi; Ishimoto, Masao; Harada, Kyuya; Suematsu, Keisuke; Abiko, Tomomi; Mochizuki, Toshihiro.
：: Theoretical and Applied Genetics, 巻 130, 番号 4, 01.04.2017, p. 743-755.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Mapping quantitative trait loci for root development under hypoxia conditions in soybean (Glycine max L. Merr.)
AU - Van Nguyen, Loc
AU - Takahashi, Ryoji
AU - Githiri, Stephen Mwangi
AU - Rodriguez, Tito O.
AU - Tsutsumi, Nobuko
AU - Kajihara, Sayuri
AU - Sayama, Takasi
AU - Ishimoto, Masao
AU - Harada, Kyuya
AU - Suematsu, Keisuke
AU - Abiko, Tomomi
AU - Mochizuki, Toshihiro
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Key message: Greatest potential, QTLs for hypoxia and waterlogging tolerance in soybean roots were detected using a new phenotypic evaluation method. Abstract: Waterlogging is a major environmental stress limiting soybean yield in wet parts of the world. Root development is an important indicator of hypoxia tolerance in soybean. However, little is known about the genetic control of root development under hypoxia. This study was conducted to identify quantitative trait loci (QTLs) responsible for root development under hypoxia. Recombinant inbred lines (RILs) developed from a cross between a hypoxia-sensitive cultivar, Tachinagaha, and a tolerant landrace, Iyodaizu, were used. Seedlings were subjected to hypoxia, and root development was evaluated with the value change in root traits between after and before treatments. We found 230 polymorphic markers spanning 2519.2 cM distributed on all 20 chromosomes (Chrs.). Using these, we found 11 QTLs for root length (RL), root length development (RLD), root surface area (RSA), root surface area development (RSAD), root diameter (RD), and change in average root diameter (CARD) on Chrs. 11, 12, 13 and 14, and 7 QTLs for hypoxia tolerance of these root traits. These included QTLs for RLD and RSAD between markers Satt052 and Satt302 on Chr. 12, which are important markers of hypoxia tolerance in soybean; those QTLs were stable between 2 years. To validate the QTLs, we developed a near-isogenic line with the QTL region derived from Iyodaizu. The line performed well under both hypoxia and waterlogging, suggesting that the region contains one or more genes with large effects on root development. These findings may be useful for fine mapping and positional cloning of gene responsible for root development under hypoxia.
AB - Key message: Greatest potential, QTLs for hypoxia and waterlogging tolerance in soybean roots were detected using a new phenotypic evaluation method. Abstract: Waterlogging is a major environmental stress limiting soybean yield in wet parts of the world. Root development is an important indicator of hypoxia tolerance in soybean. However, little is known about the genetic control of root development under hypoxia. This study was conducted to identify quantitative trait loci (QTLs) responsible for root development under hypoxia. Recombinant inbred lines (RILs) developed from a cross between a hypoxia-sensitive cultivar, Tachinagaha, and a tolerant landrace, Iyodaizu, were used. Seedlings were subjected to hypoxia, and root development was evaluated with the value change in root traits between after and before treatments. We found 230 polymorphic markers spanning 2519.2 cM distributed on all 20 chromosomes (Chrs.). Using these, we found 11 QTLs for root length (RL), root length development (RLD), root surface area (RSA), root surface area development (RSAD), root diameter (RD), and change in average root diameter (CARD) on Chrs. 11, 12, 13 and 14, and 7 QTLs for hypoxia tolerance of these root traits. These included QTLs for RLD and RSAD between markers Satt052 and Satt302 on Chr. 12, which are important markers of hypoxia tolerance in soybean; those QTLs were stable between 2 years. To validate the QTLs, we developed a near-isogenic line with the QTL region derived from Iyodaizu. The line performed well under both hypoxia and waterlogging, suggesting that the region contains one or more genes with large effects on root development. These findings may be useful for fine mapping and positional cloning of gene responsible for root development under hypoxia.
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UR - http://www.scopus.com/inward/citedby.url?scp=85009775858&partnerID=8YFLogxK
U2 - 10.1007/s00122-016-2847-3
DO - 10.1007/s00122-016-2847-3
M3 - Article
C2 - 28097398
AN - SCOPUS:85009775858
VL - 130
SP - 743
EP - 755
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
SN - 0040-5752
IS - 4
ER -