Mapping quantitative trait loci for root development under hypoxia conditions in soybean (Glycine max L. Merr.)

Loc Van Nguyen, Ryoji Takahashi, Stephen Mwangi Githiri, Tito O. Rodriguez, Nobuko Tsutsumi, Sayuri Kajihara, Takasi Sayama, Masao Ishimoto, Kyuya Harada, Keisuke Suematsu, Tomomi Abiko, Toshihiro Mochizuki

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Key message: Greatest potential, QTLs for hypoxia and waterlogging tolerance in soybean roots were detected using a new phenotypic evaluation method. Abstract: Waterlogging is a major environmental stress limiting soybean yield in wet parts of the world. Root development is an important indicator of hypoxia tolerance in soybean. However, little is known about the genetic control of root development under hypoxia. This study was conducted to identify quantitative trait loci (QTLs) responsible for root development under hypoxia. Recombinant inbred lines (RILs) developed from a cross between a hypoxia-sensitive cultivar, Tachinagaha, and a tolerant landrace, Iyodaizu, were used. Seedlings were subjected to hypoxia, and root development was evaluated with the value change in root traits between after and before treatments. We found 230 polymorphic markers spanning 2519.2 cM distributed on all 20 chromosomes (Chrs.). Using these, we found 11 QTLs for root length (RL), root length development (RLD), root surface area (RSA), root surface area development (RSAD), root diameter (RD), and change in average root diameter (CARD) on Chrs. 11, 12, 13 and 14, and 7 QTLs for hypoxia tolerance of these root traits. These included QTLs for RLD and RSAD between markers Satt052 and Satt302 on Chr. 12, which are important markers of hypoxia tolerance in soybean; those QTLs were stable between 2 years. To validate the QTLs, we developed a near-isogenic line with the QTL region derived from Iyodaizu. The line performed well under both hypoxia and waterlogging, suggesting that the region contains one or more genes with large effects on root development. These findings may be useful for fine mapping and positional cloning of gene responsible for root development under hypoxia.

元の言語英語
ページ(範囲)743-755
ページ数13
ジャーナルTheoretical and Applied Genetics
130
発行部数4
DOI
出版物ステータス出版済み - 4 1 2017

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Quantitative Trait Loci
Soybeans
hypoxia
Glycine max
quantitative trait loci
soybeans
flooded conditions
surface area
Hypoxia
Chromosomes, Human, Pair 20
isogenic lines
Seedlings
landraces
inbred lines
Genes
Organism Cloning
molecular cloning
genes
chromosomes
seedlings

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

これを引用

Mapping quantitative trait loci for root development under hypoxia conditions in soybean (Glycine max L. Merr.). / Van Nguyen, Loc; Takahashi, Ryoji; Githiri, Stephen Mwangi; Rodriguez, Tito O.; Tsutsumi, Nobuko; Kajihara, Sayuri; Sayama, Takasi; Ishimoto, Masao; Harada, Kyuya; Suematsu, Keisuke; Abiko, Tomomi; Mochizuki, Toshihiro.

:: Theoretical and Applied Genetics, 巻 130, 番号 4, 01.04.2017, p. 743-755.

研究成果: ジャーナルへの寄稿記事

Van Nguyen, L, Takahashi, R, Githiri, SM, Rodriguez, TO, Tsutsumi, N, Kajihara, S, Sayama, T, Ishimoto, M, Harada, K, Suematsu, K, Abiko, T & Mochizuki, T 2017, 'Mapping quantitative trait loci for root development under hypoxia conditions in soybean (Glycine max L. Merr.)', Theoretical and Applied Genetics, 巻. 130, 番号 4, pp. 743-755. https://doi.org/10.1007/s00122-016-2847-3
Van Nguyen, Loc ; Takahashi, Ryoji ; Githiri, Stephen Mwangi ; Rodriguez, Tito O. ; Tsutsumi, Nobuko ; Kajihara, Sayuri ; Sayama, Takasi ; Ishimoto, Masao ; Harada, Kyuya ; Suematsu, Keisuke ; Abiko, Tomomi ; Mochizuki, Toshihiro. / Mapping quantitative trait loci for root development under hypoxia conditions in soybean (Glycine max L. Merr.). :: Theoretical and Applied Genetics. 2017 ; 巻 130, 番号 4. pp. 743-755.
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abstract = "Key message: Greatest potential, QTLs for hypoxia and waterlogging tolerance in soybean roots were detected using a new phenotypic evaluation method. Abstract: Waterlogging is a major environmental stress limiting soybean yield in wet parts of the world. Root development is an important indicator of hypoxia tolerance in soybean. However, little is known about the genetic control of root development under hypoxia. This study was conducted to identify quantitative trait loci (QTLs) responsible for root development under hypoxia. Recombinant inbred lines (RILs) developed from a cross between a hypoxia-sensitive cultivar, Tachinagaha, and a tolerant landrace, Iyodaizu, were used. Seedlings were subjected to hypoxia, and root development was evaluated with the value change in root traits between after and before treatments. We found 230 polymorphic markers spanning 2519.2 cM distributed on all 20 chromosomes (Chrs.). Using these, we found 11 QTLs for root length (RL), root length development (RLD), root surface area (RSA), root surface area development (RSAD), root diameter (RD), and change in average root diameter (CARD) on Chrs. 11, 12, 13 and 14, and 7 QTLs for hypoxia tolerance of these root traits. These included QTLs for RLD and RSAD between markers Satt052 and Satt302 on Chr. 12, which are important markers of hypoxia tolerance in soybean; those QTLs were stable between 2 years. To validate the QTLs, we developed a near-isogenic line with the QTL region derived from Iyodaizu. The line performed well under both hypoxia and waterlogging, suggesting that the region contains one or more genes with large effects on root development. These findings may be useful for fine mapping and positional cloning of gene responsible for root development under hypoxia.",
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AU - Van Nguyen, Loc

AU - Takahashi, Ryoji

AU - Githiri, Stephen Mwangi

AU - Rodriguez, Tito O.

AU - Tsutsumi, Nobuko

AU - Kajihara, Sayuri

AU - Sayama, Takasi

AU - Ishimoto, Masao

AU - Harada, Kyuya

AU - Suematsu, Keisuke

AU - Abiko, Tomomi

AU - Mochizuki, Toshihiro

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N2 - Key message: Greatest potential, QTLs for hypoxia and waterlogging tolerance in soybean roots were detected using a new phenotypic evaluation method. Abstract: Waterlogging is a major environmental stress limiting soybean yield in wet parts of the world. Root development is an important indicator of hypoxia tolerance in soybean. However, little is known about the genetic control of root development under hypoxia. This study was conducted to identify quantitative trait loci (QTLs) responsible for root development under hypoxia. Recombinant inbred lines (RILs) developed from a cross between a hypoxia-sensitive cultivar, Tachinagaha, and a tolerant landrace, Iyodaizu, were used. Seedlings were subjected to hypoxia, and root development was evaluated with the value change in root traits between after and before treatments. We found 230 polymorphic markers spanning 2519.2 cM distributed on all 20 chromosomes (Chrs.). Using these, we found 11 QTLs for root length (RL), root length development (RLD), root surface area (RSA), root surface area development (RSAD), root diameter (RD), and change in average root diameter (CARD) on Chrs. 11, 12, 13 and 14, and 7 QTLs for hypoxia tolerance of these root traits. These included QTLs for RLD and RSAD between markers Satt052 and Satt302 on Chr. 12, which are important markers of hypoxia tolerance in soybean; those QTLs were stable between 2 years. To validate the QTLs, we developed a near-isogenic line with the QTL region derived from Iyodaizu. The line performed well under both hypoxia and waterlogging, suggesting that the region contains one or more genes with large effects on root development. These findings may be useful for fine mapping and positional cloning of gene responsible for root development under hypoxia.

AB - Key message: Greatest potential, QTLs for hypoxia and waterlogging tolerance in soybean roots were detected using a new phenotypic evaluation method. Abstract: Waterlogging is a major environmental stress limiting soybean yield in wet parts of the world. Root development is an important indicator of hypoxia tolerance in soybean. However, little is known about the genetic control of root development under hypoxia. This study was conducted to identify quantitative trait loci (QTLs) responsible for root development under hypoxia. Recombinant inbred lines (RILs) developed from a cross between a hypoxia-sensitive cultivar, Tachinagaha, and a tolerant landrace, Iyodaizu, were used. Seedlings were subjected to hypoxia, and root development was evaluated with the value change in root traits between after and before treatments. We found 230 polymorphic markers spanning 2519.2 cM distributed on all 20 chromosomes (Chrs.). Using these, we found 11 QTLs for root length (RL), root length development (RLD), root surface area (RSA), root surface area development (RSAD), root diameter (RD), and change in average root diameter (CARD) on Chrs. 11, 12, 13 and 14, and 7 QTLs for hypoxia tolerance of these root traits. These included QTLs for RLD and RSAD between markers Satt052 and Satt302 on Chr. 12, which are important markers of hypoxia tolerance in soybean; those QTLs were stable between 2 years. To validate the QTLs, we developed a near-isogenic line with the QTL region derived from Iyodaizu. The line performed well under both hypoxia and waterlogging, suggesting that the region contains one or more genes with large effects on root development. These findings may be useful for fine mapping and positional cloning of gene responsible for root development under hypoxia.

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