Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching

Tatsuo Maruyama, Toshimitsu Shinohara, Takuya Hosogi, Hirofumi Ichinose, Noriho Kamiya, Masahiro Goto

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Guanine quenching of a fluorescence-labeled DNA probe is a powerful tool for detecting a mutation in a targeted site of a DNA strand. However, a different guanine adjacent to a targeted site can interfere with detection of a point mutation, resulting in unsatisfactory sensitivity. In the current study, we developed a simple method to improve sensitivity of the guanine quenching method using a masking DNA oligonucleotide. The simple addition of a masking DNA oligonucleotide was found to mask the interference of a different guanine in a target oligonucleotide on fluorescence and to enhance difference in the quenching ratio between wild-type and mutant oligonucleotides. Based on this strategy, we succeeded in discriminating various mutations from the wild-type YMDD motif of the hepatitis B virus DNA polymerase gene using guanine quenching with a masking oligonucleotide.

元の言語英語
ページ(範囲)8-14
ページ数7
ジャーナルAnalytical Biochemistry
354
発行部数1
DOI
出版物ステータス出版済み - 7 1 2006
外部発表Yes

Fingerprint

Guanine
Oligonucleotides
Quenching
Mutation
DNA
Fluorescence
DNA Probes
DNA-Directed DNA Polymerase
Masks
Viruses
Point Mutation
Hepatitis B virus
Genes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching. / Maruyama, Tatsuo; Shinohara, Toshimitsu; Hosogi, Takuya; Ichinose, Hirofumi; Kamiya, Noriho; Goto, Masahiro.

:: Analytical Biochemistry, 巻 354, 番号 1, 01.07.2006, p. 8-14.

研究成果: ジャーナルへの寄稿記事

@article{8bec3b3a2b504600b2453dfb8155e01e,
title = "Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching",
abstract = "Guanine quenching of a fluorescence-labeled DNA probe is a powerful tool for detecting a mutation in a targeted site of a DNA strand. However, a different guanine adjacent to a targeted site can interfere with detection of a point mutation, resulting in unsatisfactory sensitivity. In the current study, we developed a simple method to improve sensitivity of the guanine quenching method using a masking DNA oligonucleotide. The simple addition of a masking DNA oligonucleotide was found to mask the interference of a different guanine in a target oligonucleotide on fluorescence and to enhance difference in the quenching ratio between wild-type and mutant oligonucleotides. Based on this strategy, we succeeded in discriminating various mutations from the wild-type YMDD motif of the hepatitis B virus DNA polymerase gene using guanine quenching with a masking oligonucleotide.",
author = "Tatsuo Maruyama and Toshimitsu Shinohara and Takuya Hosogi and Hirofumi Ichinose and Noriho Kamiya and Masahiro Goto",
year = "2006",
month = "7",
day = "1",
doi = "10.1016/j.ab.2006.03.056",
language = "English",
volume = "354",
pages = "8--14",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching

AU - Maruyama, Tatsuo

AU - Shinohara, Toshimitsu

AU - Hosogi, Takuya

AU - Ichinose, Hirofumi

AU - Kamiya, Noriho

AU - Goto, Masahiro

PY - 2006/7/1

Y1 - 2006/7/1

N2 - Guanine quenching of a fluorescence-labeled DNA probe is a powerful tool for detecting a mutation in a targeted site of a DNA strand. However, a different guanine adjacent to a targeted site can interfere with detection of a point mutation, resulting in unsatisfactory sensitivity. In the current study, we developed a simple method to improve sensitivity of the guanine quenching method using a masking DNA oligonucleotide. The simple addition of a masking DNA oligonucleotide was found to mask the interference of a different guanine in a target oligonucleotide on fluorescence and to enhance difference in the quenching ratio between wild-type and mutant oligonucleotides. Based on this strategy, we succeeded in discriminating various mutations from the wild-type YMDD motif of the hepatitis B virus DNA polymerase gene using guanine quenching with a masking oligonucleotide.

AB - Guanine quenching of a fluorescence-labeled DNA probe is a powerful tool for detecting a mutation in a targeted site of a DNA strand. However, a different guanine adjacent to a targeted site can interfere with detection of a point mutation, resulting in unsatisfactory sensitivity. In the current study, we developed a simple method to improve sensitivity of the guanine quenching method using a masking DNA oligonucleotide. The simple addition of a masking DNA oligonucleotide was found to mask the interference of a different guanine in a target oligonucleotide on fluorescence and to enhance difference in the quenching ratio between wild-type and mutant oligonucleotides. Based on this strategy, we succeeded in discriminating various mutations from the wild-type YMDD motif of the hepatitis B virus DNA polymerase gene using guanine quenching with a masking oligonucleotide.

UR - http://www.scopus.com/inward/record.url?scp=33744531012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744531012&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2006.03.056

DO - 10.1016/j.ab.2006.03.056

M3 - Article

C2 - 16701075

AN - SCOPUS:33744531012

VL - 354

SP - 8

EP - 14

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -