Mass production of entomopathogenic nematode, Heterorhabditits megidis by using micorosparger of gandong strain

Tae Wan Kim, Tae Hwan Kim, Chisa Yasunaga-Aoki, Yong Man Yu

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

In this study, the effects of microsparger on oxygen transfer velocity caused by micro aeration and reproduction of Heterorhabditis megidis in the process of using microsparger for mass culture production of entomopathogenic nematodes, Heterorhabditis megidis were examined. When ringsparger and microsparger were used in the incubator, changes in oxygen transfer coefficients (KLa) were measured. It tended to significantly increase when microsparger with small pore size was used. It seemed to be caused by increase in the contact area between air bubbles and media. On the other hand, in the case of microsparger, the KLa value was not increased, but it tended to be decreased when pore size got smaller despite the increase in aeration rate and stirring rate. In addition, Oxygen Uptake Rate (OUR) of Heterorhabditis megidis was 0.63×10-2mmol O2/L-min at appropriate incubation temperature of around 25°C, and it tended to rapidly increase. While being cultured, the number of the optimal inoculated bacteria in 4,000 showed the highest yield of 300×103. On the 9th day of incubation, the final yield of 3 stage IJ per ml was 400×103. 25μm micro-spargerwas attached to the incubator with capacity of 3 1 and Heterorhabditis megidis was cultured. As the result, Heterorhabditis megidisgrew with a high yield only when dissolved oxygen concentration fell below 20% and it was ventilated rather than continuous aeration.

元の言語英語
ページ(範囲)283-288
ページ数6
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
59
発行部数2
出版物ステータス出版済み - 8 1 2014

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Heterorhabditis megidis
entomopathogenic nematodes
aeration
Oxygen
Incubators
oxygen
Heterorhabditis
bubbles
dissolved oxygen
Reproduction
Air
Bacteria
uptake mechanisms
air
Temperature
bacteria
temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

これを引用

Mass production of entomopathogenic nematode, Heterorhabditits megidis by using micorosparger of gandong strain. / Kim, Tae Wan; Kim, Tae Hwan; Yasunaga-Aoki, Chisa; Yu, Yong Man.

:: Journal of the Faculty of Agriculture, Kyushu University, 巻 59, 番号 2, 01.08.2014, p. 283-288.

研究成果: ジャーナルへの寄稿記事

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abstract = "In this study, the effects of microsparger on oxygen transfer velocity caused by micro aeration and reproduction of Heterorhabditis megidis in the process of using microsparger for mass culture production of entomopathogenic nematodes, Heterorhabditis megidis were examined. When ringsparger and microsparger were used in the incubator, changes in oxygen transfer coefficients (KLa) were measured. It tended to significantly increase when microsparger with small pore size was used. It seemed to be caused by increase in the contact area between air bubbles and media. On the other hand, in the case of microsparger, the KLa value was not increased, but it tended to be decreased when pore size got smaller despite the increase in aeration rate and stirring rate. In addition, Oxygen Uptake Rate (OUR) of Heterorhabditis megidis was 0.63×10-2mmol O2/L-min at appropriate incubation temperature of around 25°C, and it tended to rapidly increase. While being cultured, the number of the optimal inoculated bacteria in 4,000 showed the highest yield of 300×103. On the 9th day of incubation, the final yield of 3 stage IJ per ml was 400×103. 25μm micro-spargerwas attached to the incubator with capacity of 3 1 and Heterorhabditis megidis was cultured. As the result, Heterorhabditis megidisgrew with a high yield only when dissolved oxygen concentration fell below 20{\%} and it was ventilated rather than continuous aeration.",
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