抄録
In this study, the effects of microsparger on oxygen transfer velocity caused by micro aeration and reproduction of Heterorhabditis megidis in the process of using microsparger for mass culture production of entomopathogenic nematodes, Heterorhabditis megidis were examined. When ringsparger and microsparger were used in the incubator, changes in oxygen transfer coefficients (KLa) were measured. It tended to significantly increase when microsparger with small pore size was used. It seemed to be caused by increase in the contact area between air bubbles and media. On the other hand, in the case of microsparger, the KLa value was not increased, but it tended to be decreased when pore size got smaller despite the increase in aeration rate and stirring rate. In addition, Oxygen Uptake Rate (OUR) of Heterorhabditis megidis was 0.63×10-2mmol O2/L-min at appropriate incubation temperature of around 25°C, and it tended to rapidly increase. While being cultured, the number of the optimal inoculated bacteria in 4,000 showed the highest yield of 300×103. On the 9th day of incubation, the final yield of 3 stage IJ per ml was 400×103. 25μm micro-spargerwas attached to the incubator with capacity of 3 1 and Heterorhabditis megidis was cultured. As the result, Heterorhabditis megidisgrew with a high yield only when dissolved oxygen concentration fell below 20% and it was ventilated rather than continuous aeration.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 283-288 |
| ページ数 | 6 |
| ジャーナル | Journal of the Faculty of Agriculture, Kyushu University |
| 巻 | 59 |
| 発行部数 | 2 |
| 出版物ステータス | 出版済み - 8 1 2014 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Agronomy and Crop Science
これを引用
Mass production of entomopathogenic nematode, Heterorhabditits megidis by using micorosparger of gandong strain. / Kim, Tae Wan; Kim, Tae Hwan; Yasunaga-Aoki, Chisa; Yu, Yong Man.
：: Journal of the Faculty of Agriculture, Kyushu University, 巻 59, 番号 2, 01.08.2014, p. 283-288.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Mass production of entomopathogenic nematode, Heterorhabditits megidis by using micorosparger of gandong strain
AU - Kim, Tae Wan
AU - Kim, Tae Hwan
AU - Yasunaga-Aoki, Chisa
AU - Yu, Yong Man
PY - 2014/8/1
Y1 - 2014/8/1
N2 - In this study, the effects of microsparger on oxygen transfer velocity caused by micro aeration and reproduction of Heterorhabditis megidis in the process of using microsparger for mass culture production of entomopathogenic nematodes, Heterorhabditis megidis were examined. When ringsparger and microsparger were used in the incubator, changes in oxygen transfer coefficients (KLa) were measured. It tended to significantly increase when microsparger with small pore size was used. It seemed to be caused by increase in the contact area between air bubbles and media. On the other hand, in the case of microsparger, the KLa value was not increased, but it tended to be decreased when pore size got smaller despite the increase in aeration rate and stirring rate. In addition, Oxygen Uptake Rate (OUR) of Heterorhabditis megidis was 0.63×10-2mmol O2/L-min at appropriate incubation temperature of around 25°C, and it tended to rapidly increase. While being cultured, the number of the optimal inoculated bacteria in 4,000 showed the highest yield of 300×103. On the 9th day of incubation, the final yield of 3 stage IJ per ml was 400×103. 25μm micro-spargerwas attached to the incubator with capacity of 3 1 and Heterorhabditis megidis was cultured. As the result, Heterorhabditis megidisgrew with a high yield only when dissolved oxygen concentration fell below 20% and it was ventilated rather than continuous aeration.
AB - In this study, the effects of microsparger on oxygen transfer velocity caused by micro aeration and reproduction of Heterorhabditis megidis in the process of using microsparger for mass culture production of entomopathogenic nematodes, Heterorhabditis megidis were examined. When ringsparger and microsparger were used in the incubator, changes in oxygen transfer coefficients (KLa) were measured. It tended to significantly increase when microsparger with small pore size was used. It seemed to be caused by increase in the contact area between air bubbles and media. On the other hand, in the case of microsparger, the KLa value was not increased, but it tended to be decreased when pore size got smaller despite the increase in aeration rate and stirring rate. In addition, Oxygen Uptake Rate (OUR) of Heterorhabditis megidis was 0.63×10-2mmol O2/L-min at appropriate incubation temperature of around 25°C, and it tended to rapidly increase. While being cultured, the number of the optimal inoculated bacteria in 4,000 showed the highest yield of 300×103. On the 9th day of incubation, the final yield of 3 stage IJ per ml was 400×103. 25μm micro-spargerwas attached to the incubator with capacity of 3 1 and Heterorhabditis megidis was cultured. As the result, Heterorhabditis megidisgrew with a high yield only when dissolved oxygen concentration fell below 20% and it was ventilated rather than continuous aeration.
UR - http://www.scopus.com/inward/record.url?scp=84908202967&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908202967&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84908202967
VL - 59
SP - 283
EP - 288
JO - Journal of the Faculty of Agriculture, Kyushu University
JF - Journal of the Faculty of Agriculture, Kyushu University
SN - 0023-6152
IS - 2
ER -