Mass spectrometry-based approaches toward absolute quantitative proteomics

Keiji Kito, Takashi Ito

研究成果: ジャーナルへの寄稿評論記事

69 引用 (Scopus)

抄録

Mass spectrometry has served as a major tool for the discipline of proteomics to catalogue proteins in an unprecedented scale. With chemical and metabolic techniques for stable isotope labeling developed over the past decade, it is now routinely used as a method for relative quantification to provide valuable information on alteration of protein abundance in a proteome-wide scale. More recently, absolute or stoichiometric quantification of proteome is becoming feasible, in particular, with the development of strategies with isotope-labeled standards composed of concatenated peptides. On the other hand, remarkable progress has been also made in label-free quantification methods based on the number of identified peptides, Here we review these mass spectrometry-based approaches for absolute quantification of proteome and discuss their implications.

元の言語英語
ページ(範囲)263-274
ページ数12
ジャーナルCurrent Genomics
9
発行部数4
DOI
出版物ステータス出版済み - 6 1 2008

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Proteome
Proteomics
Mass Spectrometry
Isotope Labeling
Peptides
Isotopes
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

これを引用

Mass spectrometry-based approaches toward absolute quantitative proteomics. / Kito, Keiji; Ito, Takashi.

:: Current Genomics, 巻 9, 番号 4, 01.06.2008, p. 263-274.

研究成果: ジャーナルへの寄稿評論記事

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