Mass spectrometry-based proteomics for quantitative description of cellular events

Keiji Kito, Takashi Ito

研究成果: ジャーナルへの寄稿評論記事

3 引用 (Scopus)

抄録

For, system-level understanding of various cellular events, it is vital to identify all molecules participating in each process and understand their interactions in a quantitative manner with spatiotemporal resolution. The advent of recent proteomics approaches has enabled large scale analyses of the quantities and interactions of proteins, the most important biomolecules, in the budding yeast Saccharomyces cerevisiae. In particular, differential protein expression analysis can be achieved by mass spectrometry with stable isotope labeling either in vitro or in vivo to quantify relative difference in protein abundance. Furthermore, researchers are further developing these techniques for absolute quantification of proteins as well as their modifications and interactions, aiming to grasp more precise pictures of the underlying molecular mechanisms for the system-level understanding. Here we review recent advance of quantitative proteomic approaches, focusing on those using mass spectrometry.

元の言語英語
ページ(範囲)629-635
ページ数7
ジャーナルCurrent Genomics
5
発行部数8
DOI
出版物ステータス出版済み - 12 1 2004
外部発表Yes

Fingerprint

Proteomics
Mass Spectrometry
Proteins
Isotope Labeling
Saccharomycetales
Saccharomyces cerevisiae
Research Personnel

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

これを引用

Mass spectrometry-based proteomics for quantitative description of cellular events. / Kito, Keiji; Ito, Takashi.

:: Current Genomics, 巻 5, 番号 8, 01.12.2004, p. 629-635.

研究成果: ジャーナルへの寄稿評論記事

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