Mass spectrometry-based proteomics for quantitative description of cellular events

Keiji Kito, Takashi Ito

研究成果: Contribution to journalReview article査読

3 被引用数 (Scopus)

抄録

For, system-level understanding of various cellular events, it is vital to identify all molecules participating in each process and understand their interactions in a quantitative manner with spatiotemporal resolution. The advent of recent proteomics approaches has enabled large scale analyses of the quantities and interactions of proteins, the most important biomolecules, in the budding yeast Saccharomyces cerevisiae. In particular, differential protein expression analysis can be achieved by mass spectrometry with stable isotope labeling either in vitro or in vivo to quantify relative difference in protein abundance. Furthermore, researchers are further developing these techniques for absolute quantification of proteins as well as their modifications and interactions, aiming to grasp more precise pictures of the underlying molecular mechanisms for the system-level understanding. Here we review recent advance of quantitative proteomic approaches, focusing on those using mass spectrometry.

本文言語英語
ページ(範囲)629-635
ページ数7
ジャーナルCurrent Genomics
5
8
DOI
出版ステータス出版済み - 12 1 2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 遺伝学(臨床)

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