Mass-tag technology for monitoring of protein kinase activity using mass spectrometry

Tatsuhiko Sonoda, Syuhei Shigaki, Takeyuki Nagashima, Osamu Okitsu, Yasuhiro Kita, Masaharu Murata, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using 32P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.

元の言語英語
ページ(範囲)847-850
ページ数4
ジャーナルBioorganic and Medicinal Chemistry Letters
14
発行部数4
DOI
出版物ステータス出版済み - 2 23 2004

Fingerprint

Protein Kinases
Mass spectrometry
Mass Spectrometry
Technology
Monitoring
Assays
Radiometry
Preclinical Drug Evaluations
Screening
Phosphotransferases
Enzyme-Linked Immunosorbent Assay
Throughput
Peptides
Substrates
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

これを引用

Mass-tag technology for monitoring of protein kinase activity using mass spectrometry. / Sonoda, Tatsuhiko; Shigaki, Syuhei; Nagashima, Takeyuki; Okitsu, Osamu; Kita, Yasuhiro; Murata, Masaharu; Katayama, Yoshiki.

:: Bioorganic and Medicinal Chemistry Letters, 巻 14, 番号 4, 23.02.2004, p. 847-850.

研究成果: ジャーナルへの寄稿記事

Sonoda, Tatsuhiko ; Shigaki, Syuhei ; Nagashima, Takeyuki ; Okitsu, Osamu ; Kita, Yasuhiro ; Murata, Masaharu ; Katayama, Yoshiki. / Mass-tag technology for monitoring of protein kinase activity using mass spectrometry. :: Bioorganic and Medicinal Chemistry Letters. 2004 ; 巻 14, 番号 4. pp. 847-850.
@article{139d794700df4487adcf01a41386bdc6,
title = "Mass-tag technology for monitoring of protein kinase activity using mass spectrometry",
abstract = "Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using 32P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.",
author = "Tatsuhiko Sonoda and Syuhei Shigaki and Takeyuki Nagashima and Osamu Okitsu and Yasuhiro Kita and Masaharu Murata and Yoshiki Katayama",
year = "2004",
month = "2",
day = "23",
doi = "10.1016/j.bmcl.2003.12.022",
language = "English",
volume = "14",
pages = "847--850",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Mass-tag technology for monitoring of protein kinase activity using mass spectrometry

AU - Sonoda, Tatsuhiko

AU - Shigaki, Syuhei

AU - Nagashima, Takeyuki

AU - Okitsu, Osamu

AU - Kita, Yasuhiro

AU - Murata, Masaharu

AU - Katayama, Yoshiki

PY - 2004/2/23

Y1 - 2004/2/23

N2 - Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using 32P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.

AB - Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using 32P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.

UR - http://www.scopus.com/inward/record.url?scp=0842346038&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0842346038&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2003.12.022

DO - 10.1016/j.bmcl.2003.12.022

M3 - Article

C2 - 15012979

AN - SCOPUS:0842346038

VL - 14

SP - 847

EP - 850

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

SN - 0960-894X

IS - 4

ER -