Mass-tag technology for monitoring of protein kinase activity using mass spectrometry

Tatsuhiko Sonoda, Syuhei Shigaki, Takeyuki Nagashima, Osamu Okitsu, Yasuhiro Kita, Masaharu Murata, Yoshiki Katayama

研究成果: Contribution to journalArticle査読

13 被引用数 (Scopus)

抄録

Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using 32P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.

本文言語英語
ページ(範囲)847-850
ページ数4
ジャーナルBioorganic and Medicinal Chemistry Letters
14
4
DOI
出版ステータス出版済み - 2 23 2004

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

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