Maternal protein restriction alters the renal ptger1 DNA methylation state in SHRSP offspring

Moe Miyoshi, Masayuki Sato, Kenji Saito, Lila Otani, Katsuhiko Shirahige, Fumihito Miura, Takashi Ito, Huijuan Jia, Hisanori Kato

研究成果: ジャーナルへの寄稿記事

抄録

We previously reported that maternal protein restriction (LP) during pregnancy increases salt sensitivity in offspring using the Stroke-Prone Spontaneously Hypertensive Rat (SHRSP). In the present study, we focus on DNA methylation profiles of prostaglandin E receptor 1 gene (ptger1), which is known to be associated with hypertension. We evaluated the ptger1 DNA methylation status via bisulfite sequencing, and analyzed the expression of ptger1-related genes. The results of these analyses showed that, compared to controls, the LP-S offspring exhibited both marked ptger1 hypermethylation, and significantly increased ptger1 expression. Moreover, they also exhibited significantly decreased expression of the downstream gene epithelial Na+ channel alpha (enacα). Interestingly, LP offspring that were provided with a standard water drinking supply (W) also exhibited increased ptger1 methylation and expression. Together, these results suggest that maternal protein restriction during pregnancy modulates the renal ptger1 DNA methylation state in SHRSP offspring, and thereby likely mediates ptger1 and enacα gene expression to induce salt sensitivity.

元の言語英語
記事番号1436
ジャーナルNutrients
10
発行部数10
DOI
出版物ステータス出版済み - 10 5 2018

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Receptors, Prostaglandin E, EP1 Subtype
DNA methylation
DNA Methylation
prostaglandins
kidneys
Mothers
Kidney
receptors
Genes
Proteins
genes
proteins
Epithelial Sodium Channels
Gene Expression
gene expression
Salts
pregnancy
salts
bisulfites
Pregnancy

All Science Journal Classification (ASJC) codes

  • Food Science
  • Nutrition and Dietetics

これを引用

Miyoshi, M., Sato, M., Saito, K., Otani, L., Shirahige, K., Miura, F., ... Kato, H. (2018). Maternal protein restriction alters the renal ptger1 DNA methylation state in SHRSP offspring. Nutrients, 10(10), [1436]. https://doi.org/10.3390/nu10101436

Maternal protein restriction alters the renal ptger1 DNA methylation state in SHRSP offspring. / Miyoshi, Moe; Sato, Masayuki; Saito, Kenji; Otani, Lila; Shirahige, Katsuhiko; Miura, Fumihito; Ito, Takashi; Jia, Huijuan; Kato, Hisanori.

:: Nutrients, 巻 10, 番号 10, 1436, 05.10.2018.

研究成果: ジャーナルへの寄稿記事

Miyoshi, M, Sato, M, Saito, K, Otani, L, Shirahige, K, Miura, F, Ito, T, Jia, H & Kato, H 2018, 'Maternal protein restriction alters the renal ptger1 DNA methylation state in SHRSP offspring', Nutrients, 巻. 10, 番号 10, 1436. https://doi.org/10.3390/nu10101436
Miyoshi, Moe ; Sato, Masayuki ; Saito, Kenji ; Otani, Lila ; Shirahige, Katsuhiko ; Miura, Fumihito ; Ito, Takashi ; Jia, Huijuan ; Kato, Hisanori. / Maternal protein restriction alters the renal ptger1 DNA methylation state in SHRSP offspring. :: Nutrients. 2018 ; 巻 10, 番号 10.
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AU - Ito, Takashi

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